注射供者的肝匀浆提取液延长大鼠异位心脏移植存活时间  

Injecting the donor's liver homogenate filtrate prolonged survival time of heterotopic heart ailograft in rats

作　　者：刘小村[1] 张百萌[2] 罗琪 颜江华[4] 丘劲华[4] 卢毅卓[1] 

机构地区：[1]厦门大学医院普外科,361005 [2]中山大学附属第五医院 [3]厦门中山医院 [4]厦门大学医学院

出　　处：《中华器官移植杂志》2006年第10期603-605,共3页Chinese Journal of Organ Transplantation

基　　金：厦门大学科技创新项目基金资助(XDKJCX20041017)

摘　　要：目的探讨注射供者的肝匀浆提取液对大鼠淋巴细胞功能及大鼠异位移植心的影响。方法以Wistar大鼠为供者,SD大鼠为受者。制作Wistar大鼠的肝匀浆提取液；建立大鼠同种异体异位心脏移植模型。(1)经受者阴茎静脉注射肝匀浆提取液0．3 ml,14d后取供、受者的血液,用四甲基偶氮唑盐(MTT)法分别测定受者对同一供者和无关供者的单向混合淋巴细胞反应(MLR)。(2)心脏移植术前2h经受者阴茎静脉注射肝匀浆提取液0．3 ml。心脏移植术后分别观察受者注射同一供者和无关供者的肝匀浆提取液后移植心脏的存活时间；心脏停跳后取移植心做病理检查及免疫组织化学检测。结果受者对同一供者和无关供者的单向MLR比较,前者明显减轻,吸光度A值分别为：0．434±0．034和0．522±0．015,两组比较,差异有统计学意义(P＜0．01)。心脏移植术前,受者接受同一供者和无关供者的肝匀浆提取液后,前者移植心脏存活时间延长,分别为(38．05±17．07)d和(9．86±2．67)d,两组比较,差异有统计学意义(P＜0．01)；且前者心肌出血、坏死程度更轻,心肌组织内IgM和IgG沉积更少。结论注射同一供者的肝匀浆提取液能特异性抑制相应个体抗原引起的淋巴细胞增殖反应,减轻大鼠移植心脏的排斥反应,明显延长其存活时间。Objective To explore the effect of injecting the donor＇s liver homogenate filtrate on lymphocyte function and heterotopic heart allograft in the rats. Methods （ 1 ） Liver homogenate filtrate of Wistar rats was made. One-way mixed lymphocyte reaction （MLR） of the SD rats, which had been injected with the donors＇ liver homogenate filtrate to the donors and the third party Wistar rats was determined by means of methyl thiazolyl tetrazolium （MTT） colorimetric assay. （2） Wistar→SD rats＇ heterotopic heart transplantation model was established. The transplanted heart beating was recorded. When heart-beating stopped, the heart allografts were resected for pathological examination and immunohistochemistry. Results One-way MLR of the SD rats to the donors was the least, and their A value was 0. 434± 0. 034, which was remarkably different from that in the other groups （P〈0. 01 ）. The survival time （38. 05 ± 17. 07）d of the transplanted hearts whose donors were identical with the liver homogenate filtrate was the longest, and their hemorrhage and necroses were lessened, with less deposition of IgM and IgG. Conclusion Injecting the donor＇s liver homogenate filtrate suppressed specially the reaction of lymphocytes proliferation to the identical donor＇s antigens, lessened rejection reaction of heart allografts, and prolonged the transplanted hearts＇ beating in rats.

关 键 词：肝提取物 心脏移植 淋巴细胞 免疫耐受 

分 类 号：R654.2[医药卫生—外科学]