基于半胱氨酸/PDDA/纳米金共修饰的过氧化氢生物传感器研究  

Hydrogen peroxide biosensor based on gold nanoparticles/PDDA/ L-cysteine modified gold electrode

作　　者：高风仙[1] 袁若[1] 柴雅琴[1] 曹淑瑞[1] 唐明宇[1] 

机构地区：[1]西南大学化学化工学院,重庆市现代分析重点实验室,重庆400715

出　　处：《化学传感器》2006年第3期24-27,共4页Chemical Sensors

基　　金：教育部优秀青年教师资助计划项目;重庆市自然科学基金(CSTS-2004BB4149;2005BB4100);西南大学高新技术培育基金(XSGX02);西南师范大学博士基金(SWNUB2004021)

摘　　要：将L-半胱氨酸、聚二烯丙基二甲基氯化铵(PDDA)、纳米金及血红蛋白(Hb)自组装到金电极表面,制成了新型过氧化氢生物传感器。采用循环伏安法和计时电流法对该传感器的性能进行了详细研究。实验发现,该传感器增加了酶的吸附量,响应快、稳定性好,对H2O2表现出良好的响应特性。检测范围为4.2×10-7～3.0×10-3mol/L,检出限为1.4×10-7mol/L,并具有抗尿酸、抗坏血酸等干扰的特点。A new hydrogen peroxide biosensor has been prepared. First, L - cysteine （ L - cys） was immobilized on the gold electrode by self - assembling. Then Hb/nano - Au/PDDA was immobilized on the modified electrode by electrostatic adsorption to prepare the hydrogen peroxide biosensor. The performance of the resulting biosensor was studied in detail by means of CV and chronoamperometry. The results obtained showed that the biosensor exhibited fast response and fine stability. The linear response of the sensor to hydrogen peroxide was in the concentration range of 4.2×10^-7～3.0×10^-3 mol/L with a detection limit of 1.4 ×10^-7mol/L. In the meantime the low operating potential of the biosensor could effectively eliminate interference from common interferents, such as ascorbic acid and uric acid.

关 键 词：纳米金 过氧化氢 生物传感器 静电吸附 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]