一种新型的苯酚电化学传感器研究  

The preparation of one novel phenol biosensor

作　　者：干宁[1] 王志颖[1] 陆敏娟[1] 余杨锋[1] 

机构地区：[1]宁波市新型功能材料及其制备科学国家重点实验室,宁波大学材料与化学工程学院,浙江宁波315211

出　　处：《化学传感器》2006年第3期33-38,共6页Chemical Sensors

基　　金：国家自然科学基金(NO.60508012);浙江省科技攻关项目(No.2006C31040)资助项目

摘　　要：制备了一种L-半胱氨卟啉镍(Ⅱ)(NiL)自组装电化学苯酚传感器(NiL/Au CME)。该电极具有良好的电活性。实验表明该电极上,苯酚可被NiL催化氧化,氧化峰电流和苯酚浓度成正比,可用于对苯酚进行测定,测定过程无需再向体系中添加媒介体。该电极对苯酚表现出快速的响应(响应时间<10s)。传感器对苯酚的测定具有较宽的线性范围(2×10-7～3×10-4mol/L),检出限为1.0×10-7mol/L。测定了电极稳定性,将该电极用于地表水中苯酚含量检测,并与标准4-氨基安替比林分光光度法作了对照,结果满意。A novel amperometric phenol sensor was constructed through the covalent binding of L - cysteamine tailed porhyrin Ni（ Ⅱ ） （NIL） on a gold electrode by using the self- assembly technique. The electrode shows good electrochemical activity in pH7.0 PBS. The experiment shows that phenol could be oxidized by immobilized NiL to form a detectable product without any mediator. The phenol sensor exhibited a fast response （less than 10 seconds） . The linear range for phenol determination was from 2.0 ×10^-7 to 3×10^-4 mol/L with a detection limit of 1.0×10^-7mol/L. The stability of the biosensor was also evaluated and shows fairly stable. It＇ s applied for the determination of phenol in real natural waters and compared with the routine 4 - aminopyrine spectro - photometry. The results are satisfactory.

关 键 词：L-半胱氨卟啉镍(Ⅱ) 苯酚传感器 电催化氧化 自组装修饰Au电极 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]