体外基因扩增制备艾滋病毒核酸探针  

Preparation of Nucleic Acid Probes of Human Immunodeficiency Virus by PCR

作　　者：杨立宏[1] 项秉懿[1] 苏成芝[1] 

机构地区：[1]第四军医大学生物化学教研室

出　　处：《第四军医大学学报》1990年第6期401-404,共4页Journal of the Fourth Military Medical University

摘　　要：作者利用DNA合成仪合成艾滋病毒ARV—2前病毒序列6 310～6 328,6 666～6 684及HT_LV-Ⅲ前病毒序列6339～6374,6385～6419两对寡核苷酸引物,应用体外基因扩增(Polymerase chainreaction,PCR),以pARV-2/7A质粒为模板扩增出两个DNA片段。^(32)P标记后,以pCP10(含HBV基因)、M56(含出血热汉坦株M片段cDNA)、人染色体DNA乙型脑炎病毒(JEV)为对照,经Southern杂交及斑点杂交,证明PCR扩增制备HIV核酸探针特异性强、敏感性高。Two pairs of synthetic olignudeotide primers of HIV gene was taken from nuddc acid sequence as from 6 310 to 6 328,6 666 to 6 684 of the provirus of ARV- 2; and from 6 339 to 6 374, 6 385 to 6 419 of the provirus of HTLV -Ⅲ. Using these paired primers, two DNA segments were amplified by PCR with pARV- 2/7A as template. The DNA segments were labded with (α- 32P) - dATP and were applied into Southern and dot blot hybridisation, using the pCP10(contain HBV gene), M56 (contain Hantavirus M segment), JEV B, Human chromosome DNA as control. It is showed that the 32P - HIV nudeotide probes, amplified by PCR, are highly specific and sensitive.

关 键 词：ENV基因 艾滋病毒 核酸探针 制备 

分 类 号：R392－33[医药卫生—免疫学]