西瓜蔓枯病分子诊断技术研究  

PCR technique for rapid diagnosis of Didymella bryoniae on watermelon

作　　者：戴富明[1] 刘少华[1] 任小杰[1] 陆金萍[1] 倪秀红[2] 徐敬友[3] 

机构地区：[1]上海市农业科学院植物保护研究所上海市设施园艺重点实验室,上海201106 [2]上海市南汇区农业技术推广中心,上海201300 [3]扬州大学农学院植物保护学系,扬州225009

出　　处：《植物病理学报》2006年第5期439-445,共7页Acta Phytopathologica Sinica

基　　金：上海市科技兴农重点攻关项目(沪农科攻字2003第3-4号);上海市科技攻关重大项目(03OZ19318)

摘　　要：本文测定西瓜上的西瓜蔓枯病菌(D idym ella bryoniae)、西瓜炭疽病菌(Colletotrichum orbiculare)及西瓜枯萎病菌(Fu-sarium oxysporumf.sp.niveum)的rDNA的ITS序列,比对近缘种及西瓜上不同病菌的ITS序列同源性,设计出特异性上游引物XM-2和下游引物XM-R2。经过对XM-2/XM-R2引物的PCR扩增条件的优化,可以扩增出一条344bp的西瓜蔓枯病菌特异性DNA条带。上述方法可以检测到pg以上的蔓枯病菌基因组DNA,并且可以准确扩增出西瓜蔓枯病自然病样中特异性的DNA片段。本文建立了一项西瓜蔓枯病分子检测技术,该方法准确、快速、可靠,可用于西瓜蔓枯病田间的快速诊断。rDNA ITS sequences of Didymella bryoniae, Colletotrichum orbiculare and Fusarium oxysporium f. sp. niveum were sequenced. A pair of specific primers XM-2 and XM-R2 was designed by homology comparison of ITS sequence of D. bryoniae with closely-related species and other pathogenic fungi from watermelon and other cucurbitaceous plants. Only a 344 bp DNA band specific to D. bryoniae was amplified with XM-2/XM-R2 primers under the optimized PCR parameters. Above pg level genomic DNA of D. bryoniae could be detected and specific DNA fragments were amplified in the samples of gummy stem blight. A rapid, accurate molecular detection method developed in the study will be useful in the future diagnosis of gummy stem blight of watermelon.

关 键 词：西瓜蔓枯病 西瓜蔓枯病菌 特异性引物 PCR诊断技术 

分 类 号：S432.44[农业科学—植物病理学]