马铃薯卷叶病毒云南分离物外壳蛋白基因的克隆与序列分析  

Cloning and sequencing of coat protein gene encoding Potato leafroll virus Yunnan isolate

作　　者：丁铭[1] 方琦[1] 李婷婷[1] 张丽珍[1] 董家红[1] 李展[1] 张仲凯[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《植物病理学报》2006年第5期473-476,共4页Acta Phytopathologica Sinica

基　　金：云南省自然科学基金重点项目资助(2005C0012Z)

摘　　要：With designed specific primers based on Potato leafroll virus(PLRV) coat protein(CP) gene sequence,one PCR fragment(about 0.6 kb) was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the total RNA as template extracted from virus infected potato plant collected in Zhaotong,Yunnan province.The fragment was cloned into pGEM-T easy vector and sequenced.The sequence was compared with that of homologous gene of other PLRV isolates.The results showed it had high homology with the other isolates(the highest homology reached 99.2% of nucleic acid).Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.With designed specific primers based on Potato leafroll virus（PLRV） coat protein （CP） gene sequence, one PCR fragment （ about 0.6 kb） was amplified by reverse transcription polymerase chain reaction （RT-PCR） using the total RNA as template extracted from virus infected potato plant collected in Zhaotong, Yunnan province. The fragment was cloned into pGEM-T easy vector and sequenced. The sequence was compared with that of homologous gene of other PLRV isolates. The results showed it had high homology with the other isolates（ the highest homology reached 99.2% of nucleic acid）. Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.

关 键 词：马铃薯卷叶病毒 外壳蛋白基因 序列分析 分离物 克隆 云南 PLRV virus 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]