水稻msp1-4突变体的鉴定及其UDT1和GAMYB基因的表达分析  

Identification of the Rice(Oryza sativa L.) Mutant msp1-4 and Expression Analysis of Its UDT1 and GAMYB Genes

作　　者：王莹[1,2] 王幼芳[1] 张大兵[3] 

机构地区：[1]华东师范大学生命科学学院 [2]上海交通大学生命科学与技术学院,上海200240 [3]上海交通大学生命科学与技术学院

出　　处：《植物生理与分子生物学学报》2006年第5期527-534,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：国家"973"基础研究计划(No.2005CB120802);教育部新世纪优秀人才计划(No.NCET-04-0403);曙光学者计划(No.04SG15)资助。~~

摘　　要：通过对粳稻‘9522’辐射诱变,得到一隐性雄性不育突变体msp1-4(MULTIPLESPOROCYTE),用遗传定位方法将该基因座位定位在分子标记WY-4和WY-8之间,相距0.8cM,物理距离247kb。测序分析证明这247kb区间中的MSP1基因的编码区在第758bp到767bp之间发生了10个碱基的缺失。形态学观察结果表明该突变体和已经报告过的msp1突变体的表型基本一致。为分析水稻其它与花药发育相关的基因在msp1-4中的表达变化,用半定量RT-PCR技术检测到影响绒毡层和花粉发育的重要基因UDT1和GAMYB的表达在突变体中比在野生型水稻中低,说明这2个基因可能位于MSP1基因的下游。A rice male-sterile mutant msp1-4 （MULTIPLE SPOROCYTE） with japonica cultivar ‘9522＇ background, was obtained in M3 popu- lation treated with ^60Co γ-ray. Results of genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus. To map this locus, an F2 population was constructed from the cross between the msp1-4 （japonica） and ‘LongTeFu B＇ （indica）. This locus was mapped between the two InDel markers, WY-4 and WY-8, with physical distance of 247 kb. A deletion with 10 base pairs between 758 bp and 767 bp in MSP1 open reading frame was confirmed by sequence analysis, which led to pre-termination of MSP1 translation. Phenotype analysis of msp1-4 indicated that it was similar to the msp1 mutant. To insight the expression change of rice anther developmental genes in this mutant, semi-quantitative RT-PCR analysis was carried out. The results showed that the expression level of rice UDT1 and GAMYB were reduced in msp1-4, implying that UDT1 and GAMYB are possibly the downstream genes of MSP1 gene in rice pollen development.

关 键 词：水稻(Dryza SATIVA L.) 雄性不育 MSP1 UDT1 GAMYB 

分 类 号：S511[农业科学—作物学]