棉花GhAQP1基因克隆及其在胚珠发育中的特异表达  

Cloning of GhAQP1 Gene and Its Specific Expression during Ovule Development in Cotton

作　　者：李登弟[1] 黄耿青[1] 谭新[1] 王杰[1] 王秀兰[1] 许文亮[1] 吴雅洁[1] 汪虹[1] 李学宝[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《植物生理与分子生物学学报》2006年第5期543-550,共8页Journal Of Plant Physiology and Molecular Biology

基　　金：教育部重点科研项目(No.104130);国家重点基础研究发展计划(973)课题(No.2004CB117304);国家自然科学基金项目(No.30470930);国家高技术研究发展计划(863)课题(No.2005AA220270)资助。~~

摘　　要：从棉花cDNA文库中筛选分离了1个编码PIP1类水孔蛋白的cDNA序列,编码287个氨基酸,命名为GhAQP1。该基因全长2096bp,编码区内含有2个内含子,分别位于编码第五和第六跨膜螺旋的碱基序列内。Northern杂交结果显示,GhAQP1基因主要在胚珠中表达,在开花后9d的胚珠中表达量最高,表明其表达不仅具有组织特异性,而且是受胚珠发育调节的。Plant aquaporins, belonging to the MIP superfamily, are a series of transmembrane proteins that facilitate water transport through cell membranes. In this study, a cDNA clone encoding the PIP1-like protein was isolated from cotton （Gossypium hirsutum） cDNA libraries, and designated as GhAQP1 （Fig.1）. We also isolated the GhAQP1 gene from cotton genome by PCR. The gene is 2 096 bp in length, including an open reading frame （ORF） and 5＇-/3＇-untranslated regions （UTR）. It contains two introns in its ORF. The first intron is inserted between codons 209 and 210 in the fifth transmembrane helix, and another is located between codons 256 and 257 in the sixth transmembrane helix of GhAQP1, respectively （Figs.2 and 3）. Northern blot analysis showed that GhAQP1 gene is expressed specifically in 6-15 DPA ovule, and reaches a peak in 9 DPA ovule （Figs.4 and 5）, suggesting that its expression is ovule-specific and developmentally regulated in cotton.

关 键 词：棉花(Gossypium hirsutum) 胚珠发育 水孔蛋白 基因 结构分析 表达模式 

分 类 号：Q943[生物学—植物学]