氨基胍和维生素C对糖尿病大鼠血脂水平和主动脉硫酸乙酰肝素蛋白多糖表达的影响  

Effects of aminoguanidine and vitamin C on blood lipid level and expression of heparan sulfate proteoglycan of aorta in streptozotocin-induced diabetic rats

作　　者：李强翔[1] 文格波[2] 王琳娜[3] 肖扬[1] 陈梅贵[1] 张卓[1] 钟惠菊[3] 

机构地区：[1]娄底市中心医院,湖南娄底417000 [2]南华大学附属第一医院临床医学研究所,湖南衡阳421001 [3]中南大学湘雅医院,湖南长沙410008

出　　处：《中国药理学与毒理学杂志》2006年第5期387-392,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：湖南省教委资助项目(97B103)~~

摘　　要：目的观察氨基胍(Am i)与维生素C(V itC)合用是否可通过抑制糖基化和氧化应激对糖尿病大鼠主动脉起到保护作用。方法腹腔注射链脲佐菌素诱导建立1型糖尿病大鼠模型,Am i,V it C和V it C+Am i治疗组分别ig Am i 100 mg.kg-1,V itC 100 mg.kg-1或V itC 100 mg.kg-1+Am i 100 mg.kg-1,每日1次,给药16周。观察大鼠的一般状况;血糖、血清甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、糖化血红蛋白(HbA1c)和糖化低密度脂蛋白(G-LDL)水平;HE染色及免疫组织化学检测主动脉内膜硫酸乙酰肝素蛋白多糖(HSPG)表达。结果与模型组相比,Am i和V it C可增加糖尿病大鼠的体重,但对血糖水平无影响;V it C降低TG、TC和LDL水平,显著提高HDL水平,Am i及V it C明显降低HbA1c和G-LDL水平,并增强主动脉HSPG表达。Am i+V it C的治疗作用较Am i及V it C更为明显,但所有的观察指标未恢复至正常组水平。结论Am i和V it C无降血糖作用,但可通过抑制糖基化和氧化应激对糖尿病大鼠主动脉起到保护作用。AIM To observe whether the protection of aminoguanidine （Ami） combined with vitamin C （Vit C） on aorta is achieved by inhibiting oxidative stress and glycosylation in streptozotocin-induced diabetic rats. METHODS Type 1 diabetic rats, induced by ip injection of streptozotocin, were treated with ig Ami 100 mg·kg^-1 ,Vit C 100 mg·kg^-1 and Vit C 100 mg·kg^-1 ＋ Ami 100 mg·kg^-1, respectively, once daily for 16 weeks. During and after the treatment, the levels of blood sugar, triglyceride, total cholesterol, low density lipoprotein （ LDL ）, high density lipoprotein （ HDL ）, glycosylated hemoglobin （ HbA1c ） and glycosylated low density lipoprotein （GLDL） were measured. Aorta tissue morphology via HE staining was observed. Heparan sulfate proteoglycan （HSPG） expression in intima of aorta was determined by immunohistochemical method. RUSULTS Compared with the model group, Ami and Vit C had no effect on the blood sugar level, while they improved body weight. Vit C decreased serum triglyceride, cholesterol, and LDL, and increased HDL significantly. Ami and Vit C decreased levels of HbA1 c and G-LDL. The expression of HSPG in aorta was significantly augmented at the same time. Therapeutic effect of Ami ＋ Vit C was better than Ami or Vit C alone obviously, but all the observed parameters did not recover to the level of control group. CONCLUSION Ami and Vit C have no effect in decreasing blood sugar level but have certain protection on aorta by inhibiting oxidative stress and glycosylation.

关 键 词：氨基胍 维生素C 糖尿病 实验性 脂蛋 白类 主动脉 硫酸乙酰肝素蛋白多糖 

分 类 号：R96[医药卫生—药理学]