家蚕胚胎细胞系BmE-SWU2的建立及其生物学特性研究  

Establishment and characterization of an embryo cell line of Bombyx mori,BmE-SWU2

作　　者：潘敏慧[1] 陈敏[1] 丁裕斌[1] 洪锡钧[1] 鲁成[1] 

机构地区：[1]西南大学蚕学与生物技术学院

出　　处：《昆虫学报》2006年第5期719-725,共7页Acta Entomologica Sinica

基　　金：国家"973"项目(2005CB121000);国家自然科学基金项目(30471312);国家科技攻关计划(2005BA711A07)

摘　　要：家蚕反转期胚胎组织经过一年多的原代培养,建立了BmE-SWU2细胞系。该细胞系细胞呈短梭形或圆形,细胞较小,属上皮型贴壁生长细胞系;细胞生长速度快,铺平率高于80%,繁殖力强,细胞群体倍增时间为51·8h。BmE-SWU2细胞系的二倍体细胞(2n=56)比例达到89·76%,属二倍体细胞系,其染色体具有鳞翅目昆虫染色体的典型特征,中期染色体呈短杆状或颗粒状。BmE-SWU2细胞系对BmNPV高度敏感,TCID50为1·581×10-7。An embryo cell line of Bombyx mori, named BmE-SWU2, was established after one year primary culture. The cells are relatively single, short shuttle-like or round, and very small in shape. The cell line belongs to adhesive-type epithelium. The cell doubling time was 51.8 h. The ratio of duple cells was 89.76%. Thus, the cell line belongs to duple cell line. The chromosomes of BmE-SWU2 was typical of Lepidoptera, namely the chromosomes in metaphase were short pole-like or granule-like and the centromeres dispersed-type. BmE-SWU2 was more sensitive to BmNPV. The 50% tissue culture infection dose （TCID50） was 1.581×10^-7.

关 键 词：家蚕 胚胎细胞系 核型 生长曲线 病毒敏感性 

分 类 号：Q962[生物学—昆虫学]