桔小实蝇抗性品系的微卫星DNA分析  

Microsatellite DNA polymorphism in different pesticide-resistant strains of the oriental fruitfly Bactrocera dorsalis (Hendel) (Diptera:Tetriphitidae)

作　　者：潘志萍[1] 曾玲[1] 温硕洋[1] 

机构地区：[1]华南农业大学昆虫学系,广州510642

出　　处：《昆虫学报》2006年第5期874-877,共4页Acta Entomologica Sinica

基　　金：国家"973"项目子课题(2002CB111400);广东省科技计划项目(2002B2160203);广东省科技计划重大专项(2004A20401002)

摘　　要：用微卫星标记对桔小实蝇Bactrocera dorsalis(Hendel)敏感品系和3个抗性品系(敌百虫抗性品系、高效氯氰菊酯抗性品系和阿维菌素抗性品系)进行了基因组筛查。结果表明,抗性品系显示出微卫星DNA多态性,不同的抗性品系已经产生了不同的遗传变异。该结果有利于指导控制桔小实蝇抗药性产生以及抗药性的田间监控,同时在抗药性产生的遗传学机制研究方面有一定的意义。In order to detect Bactrocera dorsalis （Hendel）, the genetic change in the pesticide-resistant strains of the oriental fmitfly the genomes of a sensitive strain and three pesticide-resistant strains （TrR- resistant strain, AIR-resistant strain and AbR-resistant strain ） of the fmitfly were screened using two microsatellite DNA primers. Distinct microsatellite DNA polymorphism was detected in the four strains, revealing that genetic background had changed in the tested strains. The results may contribute to further understanding the mechanism of pesticide resistance of the fly and provide some guidance for the controlling and monitoring the appearance of pesticide resistance in the field.

关 键 词：枯小实蝇 敏感品系 抗性品系 微卫星 DNA多态性 

分 类 号：Q966[生物学—昆虫学]