促甲状腺素受体在BALB/c小鼠主要组织和器官的分布及其意义  

Distribution of thyrotrophin receptor in major organ and tissues of BALB/c mice and its significance

作　　者：吕红彬[1] 闫乃红[2] 罗清礼[1] 夏庆杰[2] 曹桂群[2] 张发强[2] 

机构地区：[1]四川大学华西医院眼科,成都610041 [2]四川大学华西医院眼疾病分子遗传研究室,成都610041

出　　处：《解剖学杂志》2006年第5期557-560,620,共5页Chinese Journal of Anatomy

基　　金：四川省科技厅重点科技项目(05JY029-105-2)

摘　　要：目的:研究正常BALB/c小鼠体内各主要组织、器官的促甲状腺素受体(thyrotrophin receptor,TSHR) mRNA的分布,为Graves病(GD)或／和甲状腺相关眼病(TAO)动物模型的制作提供病理学依据。方法:采用实时荧光定量PCR技术检测BALB/c小鼠甲状腺及腺外部分组织、器官中TSHR mRNA表达水平。结果:以甲状腺组织TSHR mRNA表达水平为标准1,呈相对高表达组织依次为肾上腺、胸腺、睾丸、卵巢及眼外肌,呈相对低表达的组织依次为肾脏、脑组织、腹壁脂肪、下颌下腺、肺脏、腹壁皮肤、眼眶脂肪、肝脏直至脾脏,胰腺、心脏及腹直肌中未见TSHR mRNA表达。结论:BALB/c小鼠体内,TSHR除了在甲状腺中表达外,还广泛存在于甲状腺外组织,且全身各处的TSHR作用机制可能不同。Objective： To investigate the distribution of thyrotrophin receptor （TSHR） in major tissues and organs of BALB/c mice, providing a pathologic basis for establishing a GD and/or TAO animal model. Methods： The real-time fluorescence quantitative PCR method was used to analyze the expression of TSHR mRNA in the thyroid gland and extrathyroidal tissues of the BALB/c mice. Results： Besides in thyroid gland, abundant TSHR mRNA expression was also detected in the suprarenal gland, thymus gland, testis, ovary and extraocular muscles; while weaker expression was found in the kidney, brain, abdominal fat tissue, submandibular gland, lung, skin of abdomen, orbital adipose tissues, liver and spleen; and no expression was found in the pancreas, heart and rectus abdominis muscle. Conclusion： Besides in the thyroid gland,, TSHR extensively exists in the extrathyroidal tissues of the BALB/c mice, and its mechanism may be different in different parts of the body.

关 键 词：促甲状腺素受体 组织 器官 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]