不同月龄大鼠脑室下区胶质细胞源性神经营养因子受体α_1的表达  

Expression of GFR-α_1 in cells of subventricular zone of different months old rats

作　　者：曾水林[1] 刘方舟[1] 杨宁[1] 雷志年[1] 王磊[1] 朱建宝[1] 李涛[1] 

机构地区：[1]东南大学神经生物学研究所,南京210009

出　　处：《解剖学杂志》2006年第5期604-607,共4页Chinese Journal of Anatomy

基　　金：江苏省自然科学基金(BK2002056)

摘　　要：目的:观察胶质细胞源性神经营养因子(GDNF)受体α1(Glial cell line-derived growth factor recepterα1, GFR-α1)在1、3、8、12月龄大鼠脑室下区(SVZ)中的表达。方法:取不同月龄大鼠SVZ行冰冻切片,采用GFRα1多克隆抗体结合5′-BrdU单克隆抗体免疫组织化学单标与双标的方法染色。结果:在不同年龄大鼠SVZ可见GFR-α1、BrdU单标与双标细胞,但主要分布在SVZ前部的背外侧部分。随着年龄的增长,3种标记细胞的数量逐渐减少,且12月龄组的减少更明显。结论:GDNF可能通过GFR-α1参与调节哺乳动物脑内SVZ细胞的增殖和分化。随着年龄的增加,GFR-α1表达逐渐减少,SVZ细胞增殖、分化的能力亦减弱。Objective： To investigate the expression of glial cell line-derived neurotrophic factor receptor α1 （GFR-α1 ） in the subventricular zone （SVZ） of different months old rats. Methods： Bromodeoxyuridine （BrdU）, GFR-α1 single-labeled and double-labeled cells were detected with immunohistochemistry in SVZ. Results： It was shown that there were GFR-α1, BrdU single-labeled and double-labeled cells in SVZ of different months old rats. These labeled cells were gradually declined along with age. Conclusion： GDNF, through GFR-α1, might have important effects on the proliferation and differentiation of cells in SVZ; as the rats become older, the effects gradually decrease.

关 键 词：脑室下区 胶质细胞源性神经营养因子受体α1 年龄 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]