诊断超声照射下微泡造影剂对绿荧光蛋白质粒在肌肉细胞内表达的作用  

Effect of microbubble contrast agent on expression of plasmid GFP in muscular cells with exposure to diagnostic ultrasound

作　　者：张超[1] 邓又斌[1] 魏欣[2] 张青萍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院超声影像科,武汉430030 [2]华中科技大学同济医学院心血管内科实验室,武汉430030

出　　处：《中华超声影像学杂志》2006年第10期787-789,共3页Chinese Journal of Ultrasonography

摘　　要：目的评价诊断超声照射下微泡造影剂对绿荧光蛋白(green fluorescent protein,GFP)质粒在Wistar大鼠胫前肌中表达的作用。方法选用200 g左右成年Wistar大鼠40只,随机分为4组,每组10只,第1组只对大鼠胫前肌注射GFP质粒;第2组对大鼠胫前肌注射GFP质粒和造影剂的混合物;第3组对大鼠胫前肌注射GFP质粒和造影剂的混合物并加超声照射;第4组为阴性对照。12 d后处死动物,取胫前肌标本,制作冰冻切片后立即在荧光显微镜下观察。计数GFP阳性表达细胞数。部分组织经甲醛固定、石蜡包埋,光镜下观察病理改变。结果第3组GFP阳性表达细胞数最多[(345±19)个],与第1组[(109±15)个]、第2组[(111±14)个]差异有统计学意义(P<0.01)。第1组、第2组间差异无统计学意义(P=0.784)。所有受检组织光镜下检查未发现出血、坏死、炎症等病理改变。结论微泡造影剂在诊断超声照射下可以促进局部注射GFP质粒在大鼠胫前肌的转染。Objective To evaluate effect of ultrasound contrast agent on expression of plasmid green fluorescent protein （GFP） in tibialis anterior muscle of Wistar rats. Methods Forty adult Wistar rats weighted about 200 g were studied. The rats were randomly divided into 4 groups. In each group, 10 rats were treated with injection of GFP plasmid in tibialis anterior muscle under ultrasound guidance, injection of the mixtures of GFP plasmid and ultrasound contrast agent, injection of the mixtures of GFP plasmid and ultrasound contrast agent with simultaneous exposure to ultrasound, injection of normal saline individually. Rats were sacrificed 12 days after ultrasound treatment for gene expression assay. The number of GFP- positive skeletal muscle fibers was counted. Hematoxylin-eosin staining was performed on separate tissue specimens for histopathologic evaluation. Results Muscle tissue exposed to ultrasound with plasmid and ultrasound contrast agent showed a increase in number of GFP-positive skeletal muscle fibers （345 ± 19） with obvious difference（ P 〈 0.01 ） to other groups （109 ± 15 and 111 ± 14, respectively）. Histologic examination revealed no evidence of inflammation, hemorrhage or necrosis. Conclusions Diagnostic ultrasound with microbubble contrast agent induced efficient gene transfer to skeletal muscle of rats after local injection of GFP plasmid.

关 键 词：超声检查 造影剂 基因转移技术 肌细胞 

分 类 号：R346[医药卫生—基础医学]