双脱甲氧基姜黄素脂质体对体外培养人肺腺癌A549细胞增殖活性的影响研究  

Effect of Liposome-encapsulated Bisdemethoxycurcumin on the Proliferation of Human Lung Adenocarcinoma Cell Line A549

作　　者：宫亮[1] 杨和平[1] 王昆[2] 李剑明[1] 周向东[1] 

机构地区：[1]第三军医大学西南医院,重庆市400038 [2]第三军医大学基础医学部病原生物学教研室,重庆市400038

出　　处：《中国药房》2006年第20期1537-1539,共3页China Pharmacy

摘　　要：目的:考察双脱甲氧基姜黄素脂质体对体外培养人肺腺癌A549细胞增殖活性的影响。方法:将双脱甲氧基姜黄素脂质体作用于体外培养人肺腺癌A549细胞,分别应用形态学观察、MTT检测、流式细胞仪检测观察其生长形态、细胞周期等生物学特性变化。结果:显微镜下可见A549细胞形态明显改变;双脱甲氧基姜黄素脂质体对A549细胞增殖有抑制作用,其IC50为12.108μg/ml;流式细胞仪显示细胞周期阻滞在S期。结论:双脱甲氧基姜黄素脂质体可抑制人肺腺癌A549细胞的增殖,在肺腺癌治疗中有一定应用前景。OBJECTIVE： To investigate the effect of liposomeencapsulated demethoxycurcumm on tne proliferauu, of human lung adenocarcinoma cell line A549. METHODS： The in vitro cultured human lung adenocarcinoma cell line A549 was treated with liposome- encapsulated Bisdemethoxycurcumin. The bionomics including morphology and cell cycle of cell line A549 were observed by morphological analysis, MTT assay and flow cytometry assay. RESULTS： Marked morphological changes were noted in cell line A549 under microscope. Liposome- encapsulated Bisdemethoxycurcumin showed inhibitory action on the growth of A549 cell lines, with IC50 at 12.108μg/ mL. The progression of cell cycle was arrested in S phase. CONCLUSION： Liposome - encapsulated Bisdemethoxycurcumin can inhibit the growth of human lung adenocarcinoma cell line A549, which has a promising future in the treatment of adenocarcinoma of lung.

关 键 词：双脱甲氧基姜黄素脂质体 人肺腺癌 A549细胞 增殖活性 

分 类 号：R965[医药卫生—药理学]