基于生物信息学分析方法设计流感病毒A的通用寡核苷酸探针  

The Design of Oligonucleotide Probe in Influenza Virus A Based on Bioinformatics

作　　者：王兴友[1] 陈杭薇[1] 李兵[1] 

机构地区：[1]北京军区总医院呼吸内科,北京100700

出　　处：《华北国防医药》2006年第5期310-312,共3页Medical Journal of Beijing Military Region

基　　金：全军"十一五"科技攻关项目基金资助(06G026)

摘　　要：目的:设计流感病毒A诊断芯片的寡核苷酸(O ligo)探针。方法:利用BLAST软件对流感病毒A的基因序列进行比对,得到有意义的特异序列;用生物学软件Array Designer4.2设计特异性高、Tm值相近、长度均一的O ligo探针。结果:获得10条30 m er的O ligo探针。结论:利用BLAST软件和生物学软件Array Designer4.2设计流感病毒A诊断芯片的探针,可为后期打印成基因芯片,用于流感病毒A的检测打下基础。Objective：To design oligonucleotide probes for microarray detection of Influenza virus Type A. Methods：By analyzing the gene of Influenza Virus A with BLAST program ,a group of specific sequences for the candidate probes were acquired. Array Designer4.2 software was applied to analyze the candidates to select the probes with high specificity,identical length and similar melting temperature（Tm）. ResuIts：l 0 oligonucleotide probes were designed. Conclusion ：Ollgonucleotide probes can be designed with BLAST program and Array Designer4.2 software,and become a foundation for depositing on gene chips as the basis of the microarray for Influenza Virus A detection.

关 键 词：流感病毒A型 生物芯片 Oligo探针 BLAST软件 

分 类 号：R373.13[医药卫生—病原生物学]