田基黄药材的质量研究  

Quality study of Hypericum japonicum Thunb.(Tianjihuang)

作　　者：彭维[1] 吴钉红[1] 杨立伟[1] 王永刚[1] 李沛波[1] 苏薇薇[1] 

机构地区：[1]中山大学广州现代中药质量研究开发中心,广州510275

出　　处：《中南药学》2006年第5期340-342,共3页Central South Pharmacy

摘　　要：目的建立田基黄药材的薄层色谱鉴别法以及其中槲皮苷和异槲皮苷HPLC含量测定方法,考察不同产地、不同采收期的药材质量。方法以槲皮苷、异槲皮苷为对照品,采用聚酰胺薄膜层析法对田基黄药材进行定性;采用高效液相色谱法,以Merck lichroCART RP-18e(250 mm×4.0 mm,5μm)为色谱柱,乙腈-磷酸盐缓冲液pH=3(18∶82)为流动相,检测波长为350 nm进行定量。结果加样回收率槲皮苷97.79%、RSD为1.53%,异槲皮苷97.23%、RSD为2.06%。结论本方法简便、快速,专属性强,分离度好,重现性好。OBJECTIVE TLC and HPLC methods were established to investigate the quality of Hypericurn japonicum Thunb. （Tianjihuang） herbs with different habitats and collection dates. METHODS Quereitrin and isoquereitrin were identified by TLC, and Quereitrin and isoquereitrin were determined by HPLC. RESULTS The sensitivity and repetition of this method were good. The recovery of quereitrin was 97.79% with RSD of 1.53%, and the recovery of isoquereitrin was 97.23% with RSD of 2.06%. CONCLUSIONS This method is simple and reprodueible.

关 键 词：槲皮苷 异槲皮苷 田基黄 聚酰胺薄膜层析法 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]