硫堇褪色光度法测定微量肝素  

COLOR-FADING-PHOTOMETRIC DETERMINATION OF MICROAMOUNTS OF HEPARIN

作　　者：徐红[1] 刘绍璞[2] 罗红群[2] 席晓岚[1] 

机构地区：[1]贵阳医学院化学教研室,贵阳550004 [2]西南师范大学环境化学研究所,重庆410715

出　　处：《理化检验（化学分册）》2006年第10期829-831,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：在pH4．10的Britton-Robinson缓冲溶液中，肝素与碱性吩噻嗪染料硫堇形成离子缔合物时，染料发生明显的褪色。体系吸光度的降低与肝素钠浓度成正比，肝素的质量浓度在0～60μg／25mL范围内符合比耳定律，摩尔吸光系数为3．58×10^6L·mol^-1·cm^-1。研究了表面活性剂和共存物质的影响，表明方法选择性好。用于肝素钠注射液效价的测定，相对标准偏差小于3％，回收率为96．5％～101．9％。In a BR buffer solution of pH 4. 10, an ion association complex was formed between heparin and thionine, resulting the color-fading of the basic dye, thionine. Decrease of the intensity of absorbance of thionine was found to be directly proportional to the concentration of heparin in the range of 0-60μg/25 mL. The value of molar absorptivity was found to be 3. 58×10^6L·mol^-1·cm^-1. Effects of surfactants and co-existing substances were studied, and the results obtained showed its acceptable selectivity. The proposed method has been applied to the determination of potency of heparin injection liquor, giving RSD＇s less than 3%, and recoveries in the range of 96. 5%-101.9%.

关 键 词：褪色光度法 肝素 硫堇 

分 类 号：O657.31[理学—分析化学]