不同遗传背景小鼠的超排与体外受精率的比较研究  

The Comparative Studies on Ova Superovulated and In Vitro Fertilization in Individual Mouse Genotypes

作　　者：赵丹凤[1] 刘丽均[2] 赵立虎[1] 张春燕[2] 狄敏[2] 郁丽丽[2] 张守纯[1] 徐平[2] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]中国科学院上海实验动物中心,上海201615

出　　处：《上海交通大学学报（农业科学版）》2006年第5期465-470,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科技发展基金项目(No.044909003;No.054909007)

摘　　要：对不同遗传背景小鼠的超数排卵效果和体外受精率进行比较研究,以探索建立不同遗传背景小鼠资源冷冻胚胎库的方法。选用35个不同遗传背景的小鼠品系(其中:7个近交系、3个同类系、3个封闭群、22个突变系)分别进行超排和体外受精,观察其超排数、异常卵率及体外受精率。结果显示各品系小鼠的平均超排卵数在11.8～38.7个之间;异常卵率为0.2%～32.2%;体外受精率15.2%～97.2%.证明不同遗传背景的小鼠超排卵数、异常卵率及体外受精率差异显著(P<0.05)。To comparative studies on ova superovulated and in vitro fertilization in various genetic background mouse strains, and to explore avenue of establishing the embryo bank in individual mouse genotypes. 35 mouse strains（7 inbred, 3 congenic 3 closed colony and 22 mutant） were obtained from Shanghai SLAC Laboratory Animal Co. Ltd. Females were superovulated and operated by IVF. The results were the proportion of females yielding ovulation varied markedly among genotypes （11.8-38.7）, abnormal oocytes rate was with a range of 0.2% to 32.2%, The average rate of fertility of individual mouse genotypes was 56.23% with a range of 15.2 % to 97.2%. Concluded the fertility rate by IVF in inbred mouse were higher than outbred, mutant, congenic and induced mutant strains, individual mouse genotypes have significant differences in the rate of fertillity （P〈0.05）.

关 键 词：品系 小鼠 超数排卵 体外受精 胚胎 

分 类 号：S814.3[农业科学—畜牧学]