淫羊藿黄酮的分离鉴定及其对前破骨细胞株增殖的影响  

Isolation and identification of flavonoids from Epimedium koreanum and their effects on proliferation of RAW 264.7 cell line

作　　者：王婷[1] 张大威[2] 张金超[2] 杨梦甦[2] 肖培根[1] 

机构地区：[1]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [2]香港城市大学深圳研究院

出　　处：《中草药》2006年第10期1458-1462,共5页Chinese Traditional and Herbal Drugs

基　　金：国家863计划资助项目(2003AA2Z2052)

摘　　要：目的 观察淫羊藿中单体黄酮的抗骨质疏松作用.方法 利用硅胶和凝胶柱色谱分离淫羊藿中单体黄酮成分,根据化合物光谱数据（ESI-MS、1H-NMR和13C-NMR）鉴定其结构,通过MTT法观察其对体外培养的前破骨细胞株RAW 264.7增殖的影响.结果 从淫羊藿地上部分的醋酸乙酯萃取物中分离得到5个化合物,分别为淫羊藿苷（Ⅰ）、宝藿苷Ⅱ（Ⅱ）、朝藿素B（Ⅲ）、宝藿苷Ⅰ（Ⅳ）及金丝桃苷（Ⅴ）.活性结果表明,0.1～100 μmol/L化合物Ⅱ对前破骨细胞RAW264.7的生长有一定程度的促进作用；浓度为0.1～100 μmol/L的化合物Ⅰ对前破骨细胞RAW 264.7的生长有一定的抑制作用,但随着浓度的升高,转而表现为轻微的促进作用；浓度为1.0和10 μmol/L的化合物Ⅲ对该细胞的增殖基本上没有什么影响,而其他浓度下,则对前破骨细胞RAW 264.7的增殖表现出明显的抑制作用；除个别浓度外,化合物Ⅳ和Ⅴ均对前破骨细胞RAW 264.7的生长表现出相当程度的抑制作用.结论 黄酮类化合物对RAW 264.7的生长是促进还是抑制依赖于其自身的化学结构以及作用浓度,淫羊藿可能是通过黄酮类化合物抑制前体破骨细胞的增殖,进而抑制前体破骨细胞分化形成破骨细胞而发挥抗骨质疏松作用的.Objective To study the antiosteoporosis activity of flavonoids isolated from Epimedium koreanum. Methods The compounds were separated by column chromatography with silica gel and Sephadex LH-20 and identified by spectral analysis （ESI-MS, 1^H-NMR, and 13^C-NMR）, respectively. MTT Assay was used to investigate the effects of the compounds on proliferation of preosteoclast RAW 264.7 cell line in vitro. Results Five compounds were isolated from the ethyl acetate extract of the aerial part of E. koreanum. Their structures were identified as icariin （ Ⅰ ）, baohuoside Ⅱ （ Ⅱ ）, epimedokoreanin B （ Ⅲ ）, baohuoside Ⅰ （ Ⅳ ）, and hyperoside （ Ⅴ ）. The results indicated that compound Ⅱ could promote the proliferation of RAW 264.7 cell line at concentrations of 0. 1—100 μmol/L. Compound Ⅰ at concentrations of 0.1—100 μmol/L could inhibit the proliferation of RAW 264.7 cell line and turned to stimulate the proliferation of RAW 264.7 cell line as concentration increased. Compound Ⅲ at concentrations of 1.0 and 10 μmol/L had no effect on the proliferation, but significantly inhibited the proliferation of RAW 264.7 cell Line at other concentrations. Except individual concentration, compounds Ⅳ and Ⅴ at concentrations of 0. 1—100 μmol/L significantly inhibited the proliferation of RAW 264.7 cell line. Conclusion The effects of flavonoids on the proliferation of RAW 264.7 cell line are bi-directional, depending on concentrations and their chemical structures. The osteoporsis may be controlled by E. koreanum through inhibiting the proliferation and differentiation of preosteoclast.

关 键 词：淫羊藿 黄酮 前破骨细胞 RAW 264.7 

分 类 号：R284.1[医药卫生—中药学]