决明子中总蒽醌苷元的大孔吸附树脂纯化工艺的研究  

Purification technology of anthraquinone aglycone from Cassia obtusifolia seeds by macroporous adsorption resin

作　　者：张晓路[1] 宋元英[2] 孟祥颖[3] 薄华本[3] 乌垠[1] 鲍永利[1] 李玉新[1] 

机构地区：[1]国家教育部农业与医药基因工程研究中心,吉林长春130024 [2]长春市人民医院,吉林长春130051 [3]东北师范大学遗传与细胞研究所,吉林长春130024

出　　处：《中草药》2006年第10期1482-1486,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30472169);国家中医药管理局资助项目(04-05ZP21);吉林省中医药管理局资助项目(2004-114);吉林省科技厅重大项目(20020502);吉林省科技攻关项目(20030905)

摘　　要：目的探索决明子蒽醌苷元的大孔吸附树脂纯化工艺。方法以决明子总蒽醌苷元质量分数和收率为主要考察指标,比较了乙醚和氯仿对蒽醌苷元提取的专属性,考察了大孔吸附树脂S-8、AB-8、X-5、NKA-12、D4020、D-101对决明子蒽醌苷元的吸附和解吸附特性以及纯化能力。结果决明子药粉采用氯仿浸提,上S-8型大孔树脂柱,水洗至中性,乙醇-5%NaOH(4∶1)洗脱,醇碱洗脱液浓缩、酸化处理得到质量分数为42.62%的蒽醌苷元。结论建立了一种蒽醌苷元的纯化工艺,为决明子中蒽醌苷元类成分的工业化生产提供依据。Objective To study the purification technology of anthraquinone aglycone from Cassiae obtusifolia seeds by macroporous adsorption resin. Methods Taking the content and the extraction rate of anthraquinone aglycone as the main standard index, the efficiency to extract anthraquinone aglycone with ether and chloroform was compared, and the adsorption and desorption characters as well as the purification capacities of six types of macroporous adsorption resins, such as S-8, AB-8, X-5, NKA-12, D4020, and D101, used to treat anthraquinone aglycone were investigated preliminarily. Results Anthraquinone aglycone with the content of 42.62% was got from C. obtusifolia by extracting with chloroform, purified with S-8 type macroporous adsorption resin, eluted to pH7 by water, then concentrating and acidificating the eluent by alcohol-5% NaOH （4 ： 1）. Conclusion A preparation and purification technology of anthraquinone aglycone has been established. It provides the supporting for the industrialized production of anthraquinone aglycone from C. obtusifolia seed.

关 键 词：决明子 蒽醌苷元 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]