川乌遗传多样性的ISSR鉴定  

ISSR Identification of genetic diversity in Aconitum carmichaeli

作　　者：罗群[1] 马丹炜[1] 王跃华[2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610066 [2]成都大学生物工程系,四川成都610081

出　　处：《中草药》2006年第10期1554-1557,共4页Chinese Traditional and Herbal Drugs

基　　金：四川省教育厅重点项目(2003A084)

摘　　要：目的应用ISSR标记技术分析川乌遗传多样性。方法对江油附子和9个野生乌头居进行了ISSR分析,探索川乌各居群间的遗传多样性。结果8个引物共扩增出98条带。其中68条具有多态性,多态位点百分率为69.39%;观测等位基因数(Na)为1.6939,有效等位基因数(Ne)为1.3715,Nei′s基因多样性指数为0.2308,Shannon信息指数(I)为0.3530;用ISSR855引物能够区分川乌的10个供试居群。结论ISSR标记适合于构建川乌的DNA指纹图谱、品种鉴定和遗传多样性分析。Objective ISSR Identification of genetic diversity in Aconitum carmichaeli by ISSR marker technique. Methods Genetic diversity between Jiangyou Radix Aconiti Lateralis Preparata and nine wild A. carmichaeli populations was determined by ISSR technique. Results Eight primers were selected to produce highly reproducible ISSR bands. Among 98 amplified bands, 68 showed polymorphism, the percentage of polymorphic bands （PPB） reached to 69.39%. Observed number of alleles （Na）, effective number of alleles （Ne）, Nei＇s gene diversity index （H）, and Shannon information index （I） were 1. 693 9,1. 371 5,0. 230 8, and 0. 353 0, respectively. A DNA profile was discovered with a single primer, ISSR 855, in which each of ten tested populations had its unique patterns and was distinguished from each other. Conclusion ISSR Method is suitable for DNA fingerprinting, identification , and genetic diversity analysis of A. carmichaeli.

关 键 词：川乌 ISSR 遗传多样性 

分 类 号：R282.7[医药卫生—中药学]