羟乙葛根素降低大鼠脑缺血再灌注损伤内皮素-1和白细胞介素-6的水平  

Reducing the levels of ET-1 and IL-6 in ischemia-reperfusion injury rats with Hydroxyethylpuerarin

作　　者：王姿颖[1] 魏欣冰[1] 张斌[1] 孙茹[1] 孙霞[1] 仲英[2] 左春旭[2] 张岫美[1] 

机构地区：[1]山东大学医学院药理学研究所,山东济南250012 [2]山东省医学科学院药物研究所,山东济南250062

出　　处：《中国生化药物杂志》2006年第5期280-282,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究羟乙葛根素对大鼠脑缺血再灌注损伤内皮素-1(ET-1)和白细胞介素-6(IL-6)的影响。方法大脑中动脉阻塞法制备大鼠局灶性脑缺血再灌注损伤模型,放免法分别测定外周血和脑组织中的ET-1和IL-6的水平,探讨羟乙葛根素神经保护作用的可能机制。结果羟乙葛根素可显著降低脑缺血1 h、再灌注48 h大鼠外周血和脑组织ET-1及IL-6的含量。结论羟乙葛根素可能通过降低脑缺血再灌注损伤后血管内皮和脑组织合成及释放内皮素,减轻IL-6等细胞因子介导的炎症反应等途径对缺血性脑损伤发挥保护作用。Purpose To investigate the effect of hydroxyethylpuerarin on the levels of ET-1 and IL-6 in focal brain ischemia-reperfusion injury rats .Methods Rats were divided into 6 groups randomly, ie. sham-operate group, ischemia-reperfusion group, hydroxyethylpuerarin 15 mg/kg, 30 mg/kg, 60 m/kg groups and nimodipine 0.2 mg/kg group. Rats were prepared with a model of focal brain ischemic injury by middle cerebral artery occlusion （MCAO）, then recovered perfusion by pulling out the suture after one hour. Each animal received drugs twice a day. Results 48 hours after ischemia followed by 48 hours reperfusion, the ET-1 and IL- 6 levels in both blood and brain tissue were significantly increased. Compared with ischemia-reperfusion group, these levels were significantly decreased in all hydroxyethylpuerarin-treated groups. Conclusion Hydroxyethylpuerarin could protect neuronal injury induced by focal brain ischemia-reperfusion, probably through decreasing the synthesis and release of ET-1 or inflammatory reaction induced by some cytokines, such as IL-6.

关 键 词：羟乙葛根素 脑缺血再灌注 内皮素-1 白细胞介素-6 细胞因子 

分 类 号：R971[医药卫生—药品] R743[医药卫生—药学]