乙酰胆碱受体亚基在脊髓空洞源性脊柱侧凸患者椎旁肌的表达及其意义  

Expression of junctional acetylcholine receptor subunits of paravertebral muscles in scoliosis associated with syringomyelia

作　　者：朱泽章[1] 邱勇[1] 王斌[1] 俞杨[1] 吴亮[1] 钱邦平[1] 朱锋[1] 马薇薇[1] 

机构地区：[1]南京大学医学院附属鼓楼医院脊柱外科,南京市210008

出　　处：《中国脊柱脊髓杂志》2006年第10期762-766,共5页Chinese Journal of Spine and Spinal Cord

基　　金：南京市科技局人才培养基金资助项目(200306066)

摘　　要：目的:测定神经肌肉接头处乙酰胆碱受体分子五聚体中亚基的表达,判断脊髓空洞源性脊柱侧凸患者椎旁肌是否存在失神经支配。方法:将2003年3月￣2004年10月行手术治疗的40例患者分为3组:脊髓空洞源性脊柱侧凸(空洞-侧凸)组20例,青少年特发性脊柱侧凸(AIS)组10例,非脊柱侧凸组10例。切取脊柱两侧椎旁肌中的部分骶棘肌组织,RT-PCR法检测其神经肌肉接头处乙酰胆碱受体α和γ亚基的表达情况。结果:所有病例均有α亚基表达。空洞-侧凸组中13例(65%)出现γ亚基阳性条带,其中侧凸凸侧γ亚基表达阳性率为55%(11/20),凹侧为40%(8/20);AIS组脊柱凹、凸两侧椎旁肌的γ亚基表达均为阴性;非脊柱侧凸组仅1例左侧椎旁肌检测到γ亚基阳性条带,其余为阴性。结论:脊髓空洞源性脊柱侧凸患者椎旁肌神经肌肉接头处乙酰胆碱受体γ亚基的异常表达,提示其椎旁肌失神经支配的存在,可能是该类脊柱侧凸发生的始动因素之一。Objective：To testify the denervation of paravertebral muscles in scoliosis associated with syringomyelia via detecting the subunits expression of junctional acetyleholine receptor.Method：All cases were divided into three groups,groop A consisted of 20 patients with scoliosis associated with syringomyelia,groop B included 10 adolescents with idiopathic scoliosis,and groop C was 10 cases without scoliosis.Bilateral biopsy of paravertebral muscles was performed during scheduled spinal surgery.The mRNA expression of junctional acetylcholine receptor（AChR） subunits were detected by RT-PCR method.Result： 13 patients（65%） in groop A had the positive expression of the mRNA for γ subunit.The positive rate of γ subunit expression was 55% on the convex side of the curve,and 40% on the concave side.The γ subunit expression was absent from all samples in groop B.In groop C,however,only one patient had the γ subunit expression on the left side.Conclusion：The γ subunit expression of junctional acetylcholine receptor is testified in scoliosis associated with syringomyelia,which suggests the presence of denervation in paravertebral muscles.The denervation of paravertebral muscles may play a role in the pathogenesis of scoliosis.

关 键 词：脊柱侧凸 脊髓空洞 椎旁肌 乙酰胆碱 受体 

分 类 号：R682.3[医药卫生—骨科学] Q74[医药卫生—外科学]