RAD16-Ⅱ的物理特性及其在神经干细胞假体构建中的应用  

Evaluation of RAD16-Ⅱ scaffold on construction of neural stem cell complex

作　　者：侯天勇[1] 伍亚民[1] 龙在云[1] 李应玉[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第三研究室,重庆市400042

出　　处：《中国脊柱脊髓杂志》2006年第10期781-784,I0003-I0004,共6页Chinese Journal of Spine and Spinal Cord

基　　金：国家自然科学基金资助项目(30171068);重庆市院士基金专项(7671)

摘　　要：目的:观察RAD16-Ⅱ的物理特性以及神经干细胞在其上黏附和增殖的情况,探讨其构建的细胞假体在脊髓损伤中可能的应用。方法:将RAD16-Ⅱ的饱和水溶液同与之等体积的1.8%的氯化钠溶液混合后静置24h,通过肉眼、光镜和扫描电镜观察其表面结构,并对其吸水性和溶液pH值的动态变化进行评价;将经鉴定的神经干细胞与其共同培养,扫描电镜下观察细胞在材料上的黏附情况,同时应用免疫组化方法评价神经干细胞在材料上的增殖和分化情况。结果:RAD16-Ⅱ在1.8%的氯化钠溶液中塑型后,肉眼和光镜下形态多变,其形状取决于塑型的容器形状;扫描电镜下其为规则的网状,网孔直径为54.00±3.24"m,纤维直径为9.00±1.57"m,网孔底部相互沟通,神经干细胞可在其上迁移和增殖。结论:RAD16-Ⅱ具有良好的物理特性,神经干细胞可在与其构建的假体中迁移和良好地增殖、分化,在脊髓损伤的修复中可能具有潜在的应用前景。Objective：To investigate the characteristics of RAD16-Ⅱ and its adherence and proliferation in heron stem cell,and its roles in spinal cord injury.Method：RAD16-Ⅱ were dissolved in the same volume of 1.8% NaCl solution.The face of RAD16-Ⅱ was observed by SEM and microscope and its hydrophilia and changes of value pH in its solution was analysed.Neural stem cells （NSCs） attaching to RAD16-Ⅱ to form cellular prostheses were identified.Then adhereance and migration of neural stem cells on the RAD16-Ⅱ were observed by SEM and cellular proliferation was evaluated by dyeing nucleus with DAP1 （4＇,6-diamidino-2- phenylindole）.Result：After RAD16-Ⅱ were shaped in 1.8% NaCl,their faces were seemed as regular net under SEM with the diameter of mesh of 54.00±3.24μm and diameter of fibra of 9.00±1.57μm.Neural stem cells＇ migrate and proliferate on the RAD16-Ⅱ scaffold was documented.Conclusion：Neural stem cells attaching to RAD16-Ⅱ pertained with wonderful characteristic which make their proliferation,differentiation and migration as normal.The cellular complex will be widely used in SCI repair.

关 键 词：RAD16-Ⅱ 神经干细胞 组织工程 脊髓损伤 修复 

分 类 号：Q813.1[生物学—生物工程]