DADS活化HL-60细胞G_2/M检查点与cyclinB1亚细胞分布的关系  

The Initiation of G_2/M Checkpoint by diallyl Disulfide Associates With Subcellular Distribution of CyclinB1 in HL-60 Cells

作　　者：曾勇智[1] 汪煜华[1] 唐力铭[1] 唐圣松[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001

出　　处：《南华大学学报（医学版）》2006年第4期475-478,共4页Journal of Nanhua University(Medical Edition)

基　　金：湖南省自然科学基金重点项目(04JJ2006)

摘　　要：目的研究二烯丙基二硫(DADS)诱导人急性髓性白血病细胞系HL-60细胞G2/M期生长阻滞的分子机制。方法采用MTT法检测DADS对细胞增殖活性的影响、流式细胞术检定细胞增殖周期、蛋白印迹法检测细胞内cyclinB1表达水平。结果DADS呈浓度依赖性抑制HL-60细胞增殖活性,且DADS(20μmol/L)与ATRA(10-6mol/L)对HL-60细胞增殖活性影响相近(P<0.01),DADS(20μmol/L)作用12 h后能引起G2/M期细胞百分数增高并达到最大值,上调cyclinB1的表达水平并诱导cy-clinB1细胞质定位。结论DADS能启动HL-60细胞G2/M检查点,它的激活与cyclinB1细胞质定位有关。Objective To explore the molecular mechanisms of G~/M checkpoint iniated by diallyl disulfide （DADS） on human acute myeloid cell lineHL- 60. Methods Cell viability was determined by MTT assay. Cell cycle was assayed by flowcytometry. The expression of cyclinB1 was measured by Western blotting. Results After treatment with DADS, the growth of HL- 60 cells were suppressed in a concentration - dependent manner and the inhibition effect of DADS（20 μmol/L） was similar to that of ATRA（10^-6mol/L） （ P 〈 0.01 ）. Incubation of HL - 60 cells with DADS（20μmol/L） for 12h can induce the activation of G2/M checkpoint and increase the expres- sion of cyclinB1 and induce cytoplasmic targeting of cyclinB1. Conclusion DADS could induce the G2/M arrest in HL- 60 ceils which was involved in cytoplasmic targeting of cyclinB1.

关 键 词：二烯丙基二硫 CYCLINB1 HL-60细胞 G2/M检查点 

分 类 号：R979.1[医药卫生—药品] R733.7[医药卫生—药学]