合成肽脂与天然磷脂混合囊泡的制备及表征  

Preparation and Evaluation of Hybrid Bilayer Vesicles Formed with a Biological Phospholipid and a Synthetic Peptide Lipid

作　　者：田文杰[1] 张素萍[2] 蒋本国[1] 金黎明[1] 陈会英[1] 范圣第[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116605 [2]大连理工大学化工学院,辽宁大连116012

出　　处：《大连民族学院学报》2006年第5期36-38,共3页Journal of Dalian Nationalities University

基　　金：国家自然科学基金(20502003);教育部留学回国人员科研启动基金;辽宁省自然科学基金(20041082);辽宁省教育厅高等学校科研基金(2004F034)资助项目

摘　　要：将天然磷脂1,2-二-十四烷基磷脂酰乙醇胺(DMPE)与合成肽脂N,N-二-十六烷基-Nα-6-三甲胺基己酰基-L-甘氨酰胺混合,制得了混合双层膜囊泡,对制备过程中的超声条件进行了探讨.用透射电子显微照相、动态光散射及差示扫描量热等手段表征了混合囊泡的形态及粒径大小,确认了混合囊泡的稳定性.Hybrid bilayer vesicles were formed by combination of dimyristylphosphatidylethanolamine as a lipid signal and a cationic synthetic lipid as a matrix. The sonication condition for preparing the hybrid bilayer vesicles was discussed. The aggregate morphology was confirmed by means of transmission electron microscopy and dynamic light scatting measurements, and the vesicles showed a phase transition from gel to hquid - crystalline state at 31.2 （C as evaluated with differential scanning calorimetry.

关 键 词：磷脂信号分子 囊泡 仿生信号转导 

分 类 号：Q811.7[生物学—生物工程]