单甲氧基聚乙二醇对大鼠胰岛的免疫修饰作用  

Immune modification of rat islet by methoxy-polyethylene glycol

作　　者：赵刚[1] 崔静[1] 黎少山[1] 殷涛[1] 胡伟国[1] 吴河水[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,湖北省武汉市430022

出　　处：《世界华人消化杂志》2006年第25期2480-2483,共4页World Chinese Journal of Digestology

基　　金：湖北省卫生厅青年科技人才资助项目;No.QIX2005-4~~

摘　　要：目的:研究单甲氧基聚乙二醇(mPEG 5000)对Wistar大鼠胰岛表面抗原的掩蔽作用及对胰岛素分泌功能的影响．方法:分离Wistar大鼠的胰岛,对照组不进行任何处理,处理组分别用200,300,400 g／L mPEG包裹,培养24 h后分别用双硫腙染色检测胰岛细胞活性、葡萄糖刺激实验检测胰岛的分泌功能改变,取SD大鼠脾淋巴细胞分别与上述各组胰岛进行混合培养,通过杀伤实验检测胰岛免疫原性．结果:与对照组相比,不同浓度mPEG包裹对大鼠胰岛活性及胰岛素分泌功能无明显影响,不同浓度处理组的胰岛细胞杀伤率均明显低于对照组(24．7％,14．8％,21．4％vs 69．5％, t=2.378,2．584,2．472,P均<0．05),但300 g／L mPEG处理组的杀伤率最低(14．8％vs 24．7％, 21．4％,t=2.285,2．383,P均<0．05)．结论:mPEG有效掩蔽大鼠胰岛表面抗原,且不影响胰岛细胞的活性及胰岛素分泌功能,具有较好的免疫修饰作用．AIM： To investigate the insulin secretion changes of rat islets after immune modification of methoxy-polyethylene glycol （mPEG）. METHODS： The islets isolated from Wistar rats were divided into control group and mPEG-treated groups （with 200, 300 and 400g/L mPEG, respectively）. After culture for 24 h, the viability of islets was detected by dithizone staining, and insulin secretion function was examined by glucose stimulation test. The islets from different groups were mixed with splenic lymphocyte from SD rats to evaluate the immunogenicity. RESULTS： The viability of islets or the insulin secretion function in the three treatment groups was not significantly different from that in the control group. However, the cytoxicity rates in mPEG-treated groups were markedly lower than those in the control group （24.7%, 14.8%, 21.4% vs 69.5%, t = 2.378, 2.584, 2.472, all P 〈 0.05）. Furthermore, the cytoxicity in the islets treated with 300 g/L mPEG was the lowest （14.8% vs 24.7%, 21.4%, t = 2.285, 2.383, both P 〈 0.05）. CONCLUSION： mPEG can mask the immunogenicity of rat islets while hardly affect the viability and insulin secretion.

关 键 词：胰岛 单甲氧基聚乙二醇 免疫修饰 

分 类 号：R587.1[医药卫生—内分泌]