荧光光度法对水解胶原蛋白与铬络合物作用的模拟研究  

Study of Simulation of Reaction of Hydrolysis Collagen and Chromium Complex Compound by Fluorescent Method

作　　者：王应红[1] 陈武勇[1] 辜海彬[1] 陈继平[1] 

机构地区：[1]皮革化学与工程教育部重点实验室(四川大学),四川成都610065

出　　处：《皮革科学与工程》2006年第5期14-16,共3页Leather Science and Engineering

基　　金：国家自然科学基金项目(批准号20476064)

摘　　要：通过荧光光度法,用硫酸铬、甲酸钠及甲酸与水解胶原作用,为认识铬鞣及铬鞣机理提供更多的证据。结果表明:水解胶原蛋白中加入硫酸铬溶液,其荧光强度在315 nm处有不同程度的降低;加入硫酸铬溶液的同时,引入相同量的甲酸钠,此时荧光猝灭更明显;在一定条件下,水解胶原的pH对水解胶原荧光猝灭最明显,这可能是pH的变化引起水解胶原疏水性变化的结果。Chromic sulfate, sodium formate and formate acid react with hydrolysis collagen were investigated by fluorescent method to get the more evidence of understanding chrome tanned mechanics. Results show fluorescence intensity at 315nm has definite quenching when hydrolysis collagen reacts with chromic sulfate ;There have more apparently quenching when hydrolysis collagen reacts with the mixture of chromic sulfate and sodium formate ;The change of pH can cause most apparently quenching of hydrolysis collagen, it may be variation of pH result in the alteration of hydrophobicity of hydrolysis collagen.

关 键 词：荧光猝灭 水解胶原 铬鞣机理 铬配合物 

分 类 号：TS512[轻工技术与工程—皮革化学与工程]