山药（Dioscorea opposite）组织培养的初步研究  

Preliminary study on the tissue culture of Dioscorea opposite

作　　者：段延碧[1] 郭华春[1] 

机构地区：[1]云南农业大学薯类作物研究所,云南昆明650201

出　　处：《西南农业学报》2006年第B09期59-62,共4页Southwest China Journal of Agricultural Sciences

基　　金：本研究受云南省科技厅资助.

摘　　要：以山药带芽茎段为外植体进行组织培养，试验结果表明：（1）用75％酒精消毒15s，再用2％NaOCl浸泡15～25min为最佳的外植体消毒方法；（2）MS＋6-BA1．0mg／L＋NAA0．2mg／L为诱导多芽体较好的培养基配方；（3）MS＋NAA0．5～1．0mg／L对诱导生根较为适宜；（4）离体条件下获得的零余子在MS＋6-BA0．2mg／L＋NAA1．0mg／L的培养基上平均出芽数能达4．0，且芽的长势较好。The explants tissue culture of cauline tip with bud of Dioscorea oppsita was studied, The results showed that： The best sterilization method was to sterilized with 2% NaOCl for 15-20 min after being treated with 75% C2H2OH for 15 s ; The optimum medium for the induction of the multiple buds was the MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 mg/L; Root could be induced in the medium of MS ＋ NAA 0.5-1.0 mg/L ; The average number of shoot produced by bulbil from the in vitro culture was 4.0 by culturing in the medium of MS ＋ 6-BA 0.2 mg/L ＋ NAA 1.0 mg/L , and the buds grew well.

关 键 词：山药 多芽体 零余子 组织培养 

分 类 号：S632.1[农业科学—蔬菜学]