川芎嗪对大鼠腹腔巨噬细胞表达VEGF和HIF-1α的影响  

Effects of tetramethylpyrazine on expression of vascular endothelial growth factor and hypoxia-induced factor-1α by rat peritoneal macrophages

作　　者：徐晓玉[1] 叶兰[2] 陈刚[3] 丰俊东[2] 陈伟海[2] 胡益勇[4] 

机构地区：[1]西南大学药学与中医药学院,重庆400715 [2]重庆医科大学药学院,重庆400016 [3]重庆工商大学药物化学与化学生物学研究中心,重庆400067 [4]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《中国中药杂志》2006年第19期1608-1611,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30070915)

摘　　要：目的:探讨川芎嗪对脂多糖(LPS)诱导的巨噬细胞分泌血管内皮生长因子(VEGF)和表达缺氧诱导因子-1α(H IF-1α)的影响。方法:原代培养大鼠腹腔巨噬细胞,给予脂多糖(LPS)刺激并加入不同浓度的川芎嗪共同培养。ELISA方法检测细胞培养液中VEGF的含量,MTT法观察巨噬细胞增殖情况。western b lot检测巨噬细胞H IF-1α的表达。结果:480,48μmol.L-1川芎嗪可抑制LPS诱导的大鼠腹腔巨噬细胞分泌VEGF,并抑制巨噬细胞表达H IF-1α,但对巨噬细胞增殖没有影响。结论:川芎嗪可抑制大鼠腹腔巨噬细胞分泌血管内皮生长因子,其机制与抑制巨噬细胞表达H IF-1α密切相关。这种作用不依赖于抑制巨噬细胞的增殖。Objective： To investigate the effects of tetramethylpyrazine on lipopolysaccharides （LPS）-induced expression of vascular endothelial growth factor （VEGF） and hypoxia-induced factor-1α （HIF-1α） in macrophages. Method： Rat peritoneal macro- phages were treated with LPS, and at the same time given different doses of tetramethylpyrazine. The secreation of VEGF was determined by ELISA test, and MTT assay was used to examine cells proliferation. Westem blot assay was used to examine the expression of HIF-1α. Result： 100 μg · mL^-1 and 10 μg · mL^-1 tetramethylpyrazine decreased the secretion of VEGF and also inhibited the expres- sion of HIF-1α by LPS-induced macrophages. But these doses of tetramethylpyrazine had no effect on the cells proliferation. Conclusion： Tetramethylpyrazine could inhibit the secretion of VEGF by LPS-induced macrophages, and the mechanism must be associated with inhibiting the expression of HIF-1α. The inhibition effect was not due to inhibition of the proliferation of macrophages.

关 键 词：缺氧诱导因子 血管内皮生长因子 川芎嗪 

分 类 号：R285.5[医药卫生—中药学]