玉米分子遗传图谱的构建  

Construction of the Molecular Linkage Map with SSR Markers in Maize

作　　者：张帆[1] 万雪琴[2] 潘光堂[1] 

机构地区：[1]四川农业大学玉米研究所,四川雅安625014 [2]四川农业大学林学系,四川雅安625014

出　　处：《玉米科学》2006年第3期6-9,共4页Journal of Maize Sciences

基　　金：国家自然科学基金项目(30370889);国家863计划项目(2003AA207070);四川省生物技术攻关项目

摘　　要：以R15(抗)和Ye478(感)为亲本配制F2分离群体并以该群体为作图群体。利用778对SSR引物对亲本R15、Ye478之间的多态性进行了检测,筛选出159对多态性SSR引物用于F2群体分析。利用这159对(20.4%)多态性标记构建玉米的遗传连锁图谱,其中有9个SSR标记没连锁上。其余150个标记分布于玉米的10条染色体上,覆盖玉米基因组1775.7cM,标记间平均距离为11.8cM。230 F2 individuals derived from the single cross between resistant R15 and susceptible Ye478 maize inbred lines, were genotyped using simple sequence repeat（SSR）. 778 SSR primers pairs were used to detect the polymorphisms between parents R15 and Ye478. 159 （20.4%） SSR primers showed polymorphisms. We constructed the molecular linkage map using the 159 SSR primers, of which 9 SSR were false linkage. The others 150 SSR markers distribute among 10 linkage groups in maize and span genome about 1 775.7 cM with average distance of 11.8 cM between markers.

关 键 词：SSR 遗传图谱 玉米 

分 类 号：S513.032[农业科学—作物学]