含CpG基序的PCV-2的ORF2基因真核表达载体的构建  

Construction of Eukaryotic Vector Bearing ORF2 of PCV-2 and Containing CpG Motifs

作　　者：曹帅[1] 李俊[2] 张祯涛[3] 赵鸿雁[1] 丛晓燕[1] 张秀美[2] 王金宝[2] 

机构地区：[1]莱阳农学院,山东青岛266109 [2]山东省农业科学院畜牧兽医研究所,山东济南250100 [3]山东省畜牧兽医总站,山东济南250100

出　　处：《动物医学进展》2006年第10期84-87,共4页Progress In Veterinary Medicine

摘　　要：通过PCR扩增得到PCV-2的ORF2基因,将其插入到了真核表达载体pVAX1中,构建了重组质粒pVAX1-ORF2。作为免疫佐剂,一段150 bp的人工合成CpG-ODN被插入到了pVAX1-OFR2的骨架结构中,构建了含有CpG-ODN的表达ORF2编码蛋白的重组质粒pVAX1-ORF2-CpG。The subject of this study was to construct a recombinant plasmid DNA, which is based on ORF2 of PCV-2（porcine circovirus type 2） and contains a synthesized CpG-ODN of 150 bp in length. ORF2 of PCV-2 and a sequence consisting of 18 copies of three kinds of CpG motifs were induced into vector pVAX1＇s MCS and backbone structure, respectively. The recombinant plasmid is supposed to be used as a DNA vaccine in the further study.

关 键 词：猪圆环病毒2型 CPG-ODN 核酸疫苗 

分 类 号：S852.659.2[农业科学—基础兽医学] Q781[农业科学—兽医学]