内皮抑素在大肠杆菌中表达、纯化及复性的研究  

The investigation on expression,purification and restore of endostatin in Escherichia coli

作　　者：何晶晶[1] 金德均[1] 刘兴汉[2] 王超[1] 李继宏[2] 

机构地区：[1]哈尔滨医科大学第二临床医学院耳鼻咽喉头颈外科 [2]哈尔滨医科大学基础医学院生物化学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2006年第5期375-378,共4页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金重点项目(D0340)

摘　　要：目的探讨重组人内皮抑素(recombinant human endostatin,rhES)表达、纯化及复性的最佳生产方案。方法活化rhES工程菌,诱导表达,提取并洗涤包涵体,然后变性、复性、浓缩。通过鸡胚尿囊膜实验(chorioallantoicmembrane,CAM)检测rhES活性。结果rhES产品纯度超过95%。CAM中rhES显著抑制新血管生长。结论本实验方法可以提取到高活性、高纯度的可溶性rhES,为大规模生产rhES奠定了基础。Objective To explore the optimal method for the expression, purification and refolding of rhES in Escherichia coli. Methods The engineered bacteria carrying rhES genes was activated and induced. Exclusion bodies were extracted and washed, and then rhES was degenerated, refolded and concentrated. The activity of rhES was examined by the experiment of CAM. Results The purity of rhES surpassed 95 %. In the experiment of CAM, it was shown that rhES could obviously keep new bloodvessels from growing. Conclusion The experiment method provided by this paper can help extract highly-activated, highly-purified and soluble rhES, which will lay a foundation for large-seale production of rhES,

关 键 词：内皮抑素 大肠杆菌 表达 纯化 复性 

分 类 号：R378.21[医药卫生—病原生物学]