药物血清对缺氧/复氧心肌细胞Ca^(2+)及NOS-NO系统的相关性研究  

The research of drug serum on calcium and NOS-NO system in cardiomyocytes injured by hypoxia and reoxgyenization

作　　者：韩笑[1] 刘建勋[1] 

机构地区：[1]中国中医研究院西苑医院实验研究中心,北京100091

出　　处：《中国药理学通报》2006年第10期1211-1214,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30572186)

摘　　要：目的探讨双参通冠方药物血清对培养心肌细胞缺氧/复氧损伤的Ca2+超载、NOS-NO系统的影响。方法培养心肌细胞,建立缺氧/复氧损伤模型。运用血清药理学方法研究双参通冠方药物血清对缺氧/复氧心肌Ca2+超载、NOS-NO系统的影响。荧光分光光度法测定心肌细胞胞质[Ca2+]i,比色法检测心肌细胞培养上清NOS活性、NO含量。结果缺氧/复氧后[Ca2+]i增高,总NOS(T-NOS)及诱导型NOS(iNOS)活性增高,双参通冠方药物血清能降低缺氧/复氧心肌细胞[Ca2+]i,并能降低T-NOS、iNOS活性和NO含量(P<0.05)。结论双参通冠方药物血清可抑制缺氧/复氧损伤时心肌细胞Ca2+超载及NOS-NO系统的激活。Aim To explore the effects of shuang shen tong guan（SSTG） drug serum on calcium and NOS-NO system in cardiomyocytes injured by hypoxia and reoxgyenization. Methods The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury. Intracellular calcium concentration was determined by Fluorospectrophotometry. NOS activity and NO contents were detected using colorimetric method. Results Intracellular calcium concentration, NOS activity and NO content were increased after hypoxia and reoxygenation injury. SSTG drug serum inhibted the upwards of intracellular calcium concentration, activity of total NOS, indusive NOS and NO content due to hypoxia/reoxygenation injury significantly （ P 〈 0.05 ）. Conclusion SSTG drug serum could inhibit calcium overload and the activation of NOS-NO system due to hypoxia and reoxygenation injury.

关 键 词：中药血清药理学 缺氧/复氧 CA^+ NOS NO 

分 类 号：R-332[医药卫生] R285