细胞外α-突触核蛋白对MES23.5多巴胺能神经细胞的促增殖作用  

Promoting Effects of Extracellular α-Synuclein on the Proliferation of MES23.5 Dopaminergic Neuronal Cells

作　　者：张晨[1] 李昕[1] 李尧华[1] 韩俊燕[1] 陈彪[1] 于顺[1] 

机构地区：[1]首都医科大学宣武医院老年医学研究所神经生物学研究室

出　　处：《首都医科大学学报》2006年第5期616-619,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(30270482;30271437;30570646;30430280);北京市自然科学基金(7022011);北京市委组织部优秀人才专项经费资助项目

摘　　要：目的研究细胞外α-突触核蛋白(-αsynuclein,-αSyn)对MES23.5多巴胺能神经细胞增殖的影响。方法细胞外施加重组人-αsyn,Western blot分析和免疫细胞化学染色观察-αSyn向细胞内的进入和分布,MTT测试神经细胞的增殖。结果Western blot和免疫细胞化学染色结果均显示,细胞外-αSyn可以进入MES23.5多巴胺神经细胞并促进其增殖,这种促进作用随剂量增加而增强。-αSyn单抗明显抑制-αSyn向细胞内转移,并阻断-αSyn促细胞增殖作用。结论细胞外-αSyn对多巴胺能神经细胞的增殖具有促进作用。Objective To study the effects of extraeellular α-synuelein （α-Syn） on the proliferation of MES23. 5 Dopaminergic Neuronal Cells. Methods The recombinant human α-Syn was extracellularly administrated to the MES23. 5 dopaminergic neuronal cells. Immunohistochemistry and Western blot analysis were used to detect the α-Syn entered into the cells. MTT assay was used to measure cell proliferation. Results Extracellularly administered α-Syn entered into the neuronal cells and significantly promoted the proliferation of the cells in a dose-dependent manner. A specific monoclonal antibody against α-Syn blocked the entry of α-Syn into the cells and the cell proliferation. Conclusion Extracellular α-Syn promotes the proliferation of dopaminergic neuronal cells.

关 键 词：Α-突触核蛋白 细胞增殖 多巴胺神经细胞 

分 类 号：R338[医药卫生—人体生理学]