以ITS1-5.8SrDNA-ITS2为标记的蓝氏贾第鞭毛虫分子系统发育研究  

Molecular phylogeny of Giardia lamblia based on ITS1-5.8 SrRNA-ITS2 sequence

作　　者：温少芳[1] 卢思奇[1] 冯宪敏[1] 王凤云[1] 

机构地区：[1]首都医科大学细胞生物学系,北京100069

出　　处：《动物学报》2006年第5期954-958,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助(No.30470243)~~

摘　　要：Giardia lamblia is a widely concerned archezoan. In order to study the phylogeny of G.lamblia, ITS(1 and 2) of ribosome gene(rDNA) and 5.8 SrDNA from various Giardia strains were amplified,cloned and sequenced. A molecular phylogenetic tree was constructed with NJ method. After analyzed by DNAMAN software, 12 bases in 2 of the 10 squences were found to be substituted and 1 of the 2 had one base deletion, while the other 8 squences were quite homologous. The constructed phylogenetic tree was consistent with the phylogenic relationship reported previously. All the results show that ITS(1 and 2) and 5.8 SrRNA genes are effective genetic markers for studies of the evolution of Giardia.Giardia lamblia is a widely concerned arehezoan. In order to study the phylogeny of G. lamblia, ITS （1 and 2） of ribosome gene （rDNA） and 5.8 SrDNA from various Giardia strains were amplified, cloned and sequenced. A molecular phylogenetic tree was constructed with NJ method. After analyzed by DNAMAN software, 12 bases in 2 of the 10 squences were fotmd to be substituted and 1 of the 2 had one base deletion, while the other 8 squences were quite homologous. The constructed phylogenetic tree was consistent with the phylogenic relationship reported previously. All the results show that ITS （1 and 2） and 5.8 SrRNA genes are effective genetic markers for studies of the evolution of G/ardia .

关 键 词：蓝氏贾第鞭毛虫 核糖体基因内转录间隔区(ITS) 5.8 SrDNA 系统发育 

分 类 号：Q951[生物学—动物学]