正常生育男性成熟精子mRNA的种类和特征  

Characterization of the mRNA Profile in Ejaculated Spermatozoa from Healthy Fertile Men

作　　者：赵仰星[1] 李巧丽[1] 王朝霞[1] 王一飞[1] 王莲芸[2] 乔中东[3] 

机构地区：[1]上海交通大学医学院,上海200240 [2]上海交通大学生命科学与技术学院,上海200240 [3]上海交通大学农业与生物学院,上海200240

出　　处：《中华男科学杂志》2006年第10期900-903,共4页National Journal of Andrology

摘　　要：目的:剖析具有正常生育能力男性成熟精子mRNA的种类和丰度。方法:健康并育有后代的成年男性10例,禁欲1周后取精液,上游法优化精子,Trizol试剂提取精子总RNA。混合所有总RNA进行基因表达系列分析(SAGE)。结果:所建SAGE文库共获877个克隆,测序得到21 052个标签,出现两次以上的独特性标签有2 712种。经与SAGEm ap数据库比对,19.7%的独特性标签没有基因匹配,代表新基因;其余能匹配的基因中,67%具有蛋白质结合或核酸结合能力,41%具有催化活性,13%与信号转导有关,与细胞运输、精子结构、转录调节相关者分别达到10%左右。结论:人成熟精子中存在数量庞大、种类繁多的mRNA。Objective： To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men. Methods： Semen sampies were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis. Results： A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively. Conclusion： There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.

关 键 词：人成熟精子 mRNA表达谱 基因表达系列分析 男性生殖 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]