正常人类精子电压依赖性钙通道类型的分子生物学鉴别  

Molecular Identification of Ca^(2+) Channels in Human Ejaculated Sperm

作　　者：李路[1] 刘继红[1] 李家贵[1] 叶章群[1] 王修来[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030 [2]南京军区南京总医院博士后科研工作站,江苏南京210002

出　　处：《中华男科学杂志》2006年第10期910-912,共3页National Journal of Andrology

基　　金：江苏省"六大人才高峰"资助项目(2005A5)

摘　　要：目的:利用分子生物学技术对正常人类精子中电压依赖性钙通道的类型进行鉴别。方法:根据WHO标准用计算机辅助精液分析筛选出5例正常精子标本(前向运动精子>60%,活率>80%),混合后用上游法对精子进行优化。采用RT-PCR检测人类精子中电压依赖性钙通道的α亚单位。结果:α1A和α1D未检测出,而其他α亚单位,如α1H、α1G、α1E、α1B、α1C均有表达。结论:在正常人类精子中电压依赖性钙通道显著表达T型或非L型RNA,在精子运动和精子顶体反应中,细胞外的钙离子内流可能是通过T型或非L型电压依赖性钙通道介导的。Objective： To identify the types of voltage-dependent Ca^2＋ channels （VDCCs） in human ejaculated sperm using molecular technology. Methods： Based on the WHO criteria, the donated semen was screened by computer-aided sperm analysis （CASA） , the sperm optimized by swlm-up method, and the VDCCs＇α1 subunits examined by reverse transcription polymerase chain reaction （RT- PCR）. Results： Such mRNA messages as α1H, α1G, α1E, α1B and α1C were detected, but α1A and α1D were not. Conclusion ： Human motile sperms express multiple VDCC RNAs, among which T-type and non-L-type channel messages are likely to be predominantly expressed. Not only T-type but also non-L-type calcium channels may be major gates for the external calcium influx, which helps regulate sperm motility and acrosome reaction.

关 键 词：电压依赖性钙通道 计算机辅助精液分析 反转录-聚合酶链式反应 人类 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]