TLC-HPLC法对归元口服液中鹿茸的鉴定  

Identification of Cornu cervi pantotrichum in Guiyuan Oral Solution by TLC-HPLC

作　　者：罗立宇[1] 张贵君[1] 李影 张佳滨 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]哈尔滨世一堂制药厂,黑龙江150088 [3]北京宏泰康达医药科技有限公司,北京100032

出　　处：《北京中医药大学学报》2006年第9期623-626,共4页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的探讨归元口服液中主要成分之一鹿茸的鉴别方法,为质量标准提供科学依据。方法定性鉴别采用TLC法,含量测定采用HPLC法。结果TLC法鉴别,对照药材、供试品有相同颜色的棕红色斑点,阴性样品无干扰。HPLC法鉴别,测定鹿茸中的成分尿苷和次黄嘌呤,用ODS2C18色谱柱,流动相[甲醇-四氢呋喃(2∶1)]-水(0.1∶99.9)检测波长254nm,尿苷回收率为99.67%,RSD=2.23%;次黄嘌呤回收率为98.18%,RSD=2.14%。结论TLC-HPLC法可以作为归元口服液中鹿茸的质量控制方法之一,处方中鹿茸的投药量不少于5.0%时,尿苷、次黄嘌呤在供试品中含量的最低浓度分别高于0.130g/L和0.050g/L。Objective To investigate the identification of cornu cervi pantotrichum in Guiyuan Oral Solution （GOS） and to provide the scientific basis for the qualitative standard control. Methods TLC was used for qualitative identification and HPLC was used for quantitative detection. Results in TLC procedure the control material and the test sample showed the same coloured spots of red brown, while the negative control sample showed no interference. In HPLC procedure the contents of uridine and hypoxantine were determined in cornu cervi pantotrichum. The chromatographic column was ODS2 C18, the mobile phase was [ methanol to tetrahydrofuran （ 2 ： 1 ） ] -water （ 0. 1 ： 99. 9 ）, and the detection wavelength was 254nm. The average recovery of uridine was 99.67% （ RSD = 2.23% ） and that of hypoxantine was 98. 18% （ RSD = 2. 14% ）. Conclusion TLC-HPLC can be used as one of the methods in the qualitative control of cornu cervi pantotrichum in GOS. When the dosage of cornu cervi pantotrichum was more than 5.0% in GOS the lowest concentration of uridine and hypoxantine in the test sample would be higher than 0. 130 g/L and 0. 050 g/L respectively.

关 键 词：薄层扫描 高效液相色谱 归元口服液 鹿茸 

分 类 号：R284.2[医药卫生—中药学]