表达降钙素基因相关肽的单纯疱疹病毒Ⅰ型扩增子载体免疫效果观察  

Immunization effect of the neurotropic herpes simplex virus type Ⅰ amplicon vector bearing CGRP gene

作　　者：吴军[1] 张海鸥[1] 陈玉丙[2] 周芬莉[1] 李珂静[1] 

机构地区：[1]北京大学深圳医院神经内科,广东深圳518036 [2]吉林大学第一临床医院血液科,吉林长春130021

出　　处：《中国实验诊断学》2006年第10期1126-1128,共3页Chinese Journal of Laboratory Diagnosis

基　　金：深圳市科技局科研基金资助(编号:200304123)

摘　　要：目的探讨重组pUPO-Δγ134.5-CGRP扩增子对免疫系统的作用。方法用重组扩增子感染BALB/C小鼠,分离血清及淋巴细胞,流式细胞仪法、免疫组化法、LDH法、分别检测外周血CD4、CD8分类、TCR、NK细胞、CTL细胞以及LAK细胞活性杀伤活性。结果重组扩增子明显增强了机体的免疫力。结论该重组扩增子具有增强机体免疫力的作用。Objective To detect immunization effect of the recombinant plasmid of pUPO-△γ134.5-CGRP, Methods CD4 and CD8 cells were detected by FCS, TCR was detected through histological chenfistry straining, NK, CTL and LAK were detected by LDH array, Results The recombinant plasmid improved the immunization. Conclusion The recombinant plasmid is valuable to designing HSV gene therapy.

关 键 词：HSV-Ⅰ 免疫 TCR 

分 类 号：R392[医药卫生—免疫学]