在烟草和蓝猪耳花粉萌发及花粉管生长中阿拉伯半乳糖蛋白的作用  

Effects of Arabinogalactan Proteins on Pollen Germination and Pollen Tube Growth of Torenia fourieri and Nicotiana tabacum L.

作　　者：赵振东[1] 秦源[1] 吴娟子[1] 赵洁[1] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2006年第5期397-402,共6页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30370089);教育部博士点专项基金(20050486015)资助

摘　　要：采用花粉离体培养技术研究了阿拉伯半乳糖蛋白(arab inogalactan prote ins,AGPs)在烟草和蓝猪耳花粉萌发及花粉管生长中的作用。结果表明:βG lcY(-βG lucosyl Yariv reagent,一种能与AGPs特异结合的试剂)处理导致蓝猪耳花粉萌发率和花粉管生长速度下降;βG lcY对烟草花粉管的生长也有一定的抑制作用。另外,采用免疫荧光技术,发现在烟草和蓝猪耳未萌发的花粉粒中,AGPs主要分布在萌发孔上;在烟草离体生长的花粉管中AGPs呈均匀分布,但是在蓝猪耳离体生长的花粉管中未观察到AGPs的分布。Effects of arabinogalactan proteins in pollen germination and pollen tube growth of Toreniafourieri and Nicotiana tabacum were studied with the in vitro pollen tube growth system. The results showed that the frequency of pollen germination and the speed of pollen tube growth of T. fourieri were decreased by the treatment of βGlcY （ β-Glucosyl Yariv reagent, a regent specifically bind AGPs）. The growth of tobacco pollen tubes was also inhibited by the provision of βGlcY to the medium. With the immunocytochemistry localization techniques, it was observed that AGPs were mainly distributed in the apertures of pollen grains. When the pollen tubes germinated, AGPs were distributed throughout the whole pollen tubes uniformly in N. tabacum L. , but absent in the pollen tubes of T. fourieri.

关 键 词：烟草 蓝猪耳 花粉和花粉管 阿拉伯半乳糖蛋白 β-Glucosyl Yariv REAGENT 

分 类 号：Q944.46[生物学—植物学]