Species-specific SCAR markers for authentication of Sinocalycanthus chinensis  

作　　者：YE Qian QIU Ying-xiong QUO Yan-qi CHEN Jian-xin YANG Shu-zhen ZHAO Ming-shui FU Cheng-xin 

机构地区：[1]Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China [2]Management Bureau of Tianmushan National Nature Conservative Area, Lin ＇an 311311, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2006年第11期868-872,共5页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. G2000046806) supported by the National Basic Research Program (973) of China

摘　　要：Sinocalycanthus chmensis, an endangered species endemic to China, is cultivated as an omamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthusfloridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR （inter-simple sequence repeats） were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR （sequence characterized amplified regions） markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs ofS. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid （results can be obtained in less than 3 h）, but is also easy to perform. Hence it is a feasible method for identifying S. chmensis in seedling market.Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.

关 键 词：AUTHENTICATION Diagnostic PCRs ISSR SCAR Sinocalycanthus chinensis Specie-specific 

分 类 号：Q789[生物学—分子生物学] Q94