脱氢表雄酮对氧化低密度脂蛋白诱导的血管平滑肌细胞中VCAM-1和MCP-1表达的影响  

Effects of Dehydroepiandrosterone on Expression of VCAM-1 and MCP-1 Induced by Oxidized Low Density Lipoprotein in Vascular Smooth Muscle Cells

作　　者：丁艳萍 阮秋荣[2] 胡晓静[2] 

机构地区：[1]洛阳东方医院,河南洛阳471003 [2]华中科技大学同济医学院

出　　处：《河南科技大学学报（医学版）》2006年第3期166-168,共3页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的 探讨脱氢表雄酮（DHEA）对氧化低密度脂蛋白诱导的血管平滑肌细胞中VCAM-1及MCP-1表达的影响，研究脱氢表雄酮在动脉粥样硬化中的作用。方法 取4～6周龄雄性SD大鼠，进行血管平滑肌细胞的原代培养，当细胞纯度达95％以上，取第3～4代细胞用于实验。按实验设计将培养细胞分为4个组：①正常对照组；②ox-LDL刺激组；③ox-LDL＋DHEA组；④DHEA组。采用逆转录聚合酶链反应（RT-PCR）检测其VCAM-1及MCP-1mRNA的表达情况。结果 逆转录聚合酶链反应结果显示，各组细胞均表达VCAM-1mRNA和MCP-1mRNA，OX-LDL组VSMC表达的VCAM-1和MCP-1mRNA明显高于正常对照组（P〈0．01），而同时加入DHEA后其mRNA表达则有下降（P〈0．05及P〈0．01）。单独加DHEA组与正常对照组比较，VCAM-1和MCP-1mRNA表达与正常组mRNA无差异。结论 OX-LDL能明显诱导VSMC中VCAM-1及MCP-1的表达，DHEA能够抑制OX-LDL诱导的VSMC细胞VCAM-1及MCP-1的分泌，可能是其抗动脉粥样硬化的机制之一。Objective To investigate the effects of dehydroepiandrosterone （DHEA）on expression of VCAM-1 and MCP-1 in vascular smooth muscle cells induced by oxidized low-density lipoprotein. To study the effects of dehydroepiandrosterone in atherosclerosis, the possible mechanism of its antiatherosclerosis. Methods Rat aortic SMCs were prepared from 4 ～ 6 week old SD rat. When the purity of cells achieved 95 %, the cells of the third or the fourth passage were used in experimentation. We separated the cells into four groups according to the experimental plan： ①control group; ② ox-LDL stimulated group; ③ox-LDL ＋ DHEA group; ④DHEA group. The expression of VCAM-1 and MCP-1 mRNA were determined by semi-quantitative reverse-transcription polymerase chain reaction （RT-PCR）. Results RT-PCR showed that VCAM-1 and MCP-1 mRNA was expressed in all groups. Higher level of VCAM-1 and MCP-1 mRNA expression induced by ox-LDL was observed in ox-LDL stimulated group compared with that in control group（ P 〈 0.01 ）. After being treated with dchydroepiandrosterone, the expression of VCAM-1 and MCP-1 mRNA in ox-LDL ＋ DHEA group was reduced（ P 〈 0.05 and P 〈 0.01 ）. The expression of VCAM-1 and MCP-1 mRNA of DHEA group had no distinctive difference compared with control group. Conclusion The mRNA in cultured VSMC is markedly induced by ox-LDL, and the expressions of VCAM-1 and MCP-1 protein and mRNA induced by ox-LDL can be inhibited by dehydroepiandrosterone. That may be one of the mechanisms of antiatherosclerotic effect of DHEA.

关 键 词：脱氢表雄酮 氧化低密度脂蛋白 逆转录聚合酶链反应 细胞间粘附分子-1 单核细胞趋化蛋白-1 

分 类 号：R392.11[医药卫生—免疫学]