肝硬化对大鼠和病人血锰等6种金属含量的影响  

Effects of liver cirrhosis on the levels of manganese and the other 5 metal elements in whole-blood of rat and patient

作　　者：陆继培[1] 宾萍[1] 姜岳明[1] 高玉秋[1] 黄力毅[2] 刘守廷[3] PIRA Enrico ZHENG Wei 

机构地区：[1]广西医科大学劳动卫生学与毒理学教研室,广西南宁530021 [2]广西医科大学第一附属医院传染病科 [3]广西分析测试研究中心 [4]意大利都灵大学创伤学,矫形学与职业医学系 [5]美国普度大学卫生科学学院

出　　处：《毒理学杂志》2006年第5期299-301,共3页Journal of Toxicology

基　　金：中意科技合作交流项目(2002-389H30);广西自然科学基金项目(0007054);广西科学研究与技术开发计划项目(0443004-42)

摘　　要：目的 探讨肝硬化对血锰（Mn）等6种金属含量的影响,为临床上阐明肝硬化所致金属元素代谢紊乱的并发症提供科学依据.方法 将20只大鼠随机分为肝硬化组和正常对照组,每组10只;另观察52例肝硬化病例及44例非肝硬化对照者,采用电感偶合等离子发射光谱法测定大鼠和病人血锰（Mn）、锌（Zn）、铜（Cu）、铁（Fe）、钙（Ca）和镁（Mg）含量.结果 肝硬化大鼠全血Mn、Zn、Cu、Ca的含量明显高于正常对照组（P＜0.05～P＜0.01）,而Fe含量明显低于正常对照组（P＜0.05）.肝硬化病人血Mn含量为（1.45±1.27）μmol/L,明显比非肝硬化病人的（0.36±0.18）μmol/L高（P＜0.01）;Zn、Fe和Mg含量分别为（74.46±17.23）、（6 507.50±1 413.03）、（1 005.83±157.50）μmol/L明显低于非肝硬化病人（P＜0.01）.结论 肝硬化时,大鼠和病人血Mn等6种金属的含量是受到不同程度的影响.因此,在临床上检测肝硬化病人血液上述金属的含量,可能有益于防止因肝硬化所致某些金属缺乏和（或）过多所引起的并发症,具有重要的临床毒理学意义.Objective To study the effects of some metal elements in whole-blood of rats and patients with liver cirrhosis. Methods Twenty rats were divided into two groups at random. Each group had ten rats. Additional 55 patients with liver cirrhosis and 44 patients with non-liver cirrhosis were observed. The concentrations of manganese（Mn） ,zinc（Zn）,copper（Cu）, ferrum（Fe）, calcium （Ca） and magnesium（Mg） in whole-blood of rats and patients were detected with inductively coupled plasma-atomic emission spectrophotometry （ICP-AES）. Results Among of liver cirrhosis rats, the concentration of Mn, Zn, Cu and Ca in the whole-blood was significantly higher than that of control group, but the concentrations of Fe in rats with liver cirrhosis were lower than that in control. The concentration of Mn was（ 1.45 ± 1.27）μmol/L in patients with liver cirrhosis,which was significantly higher than those in control. The concentrations of Zn, Fe and Mg in patients with liver cirrhosis were （74.46 ± 17.23）, （6 507.50 ± 1 413.03）, （ 1 005.83 ± 157.50） μmol/L respctively, which were lower than those in control. Conclusion The concentrations of metal elements in whole-blood of rats and patients with liver cirrhosis were affected differently during liver cirrhosis. Therefore, on clinically, we should detect the concentration of these metal elements in whole-blood, as may be beneficial to prevent the syndromes that be caused by these metal elements lack and/or overabundant root from liver cirrhosis. On a certain extent, it has some significance on the clinical toxicology.

关 键 词：肝硬化 大鼠 全血 锰 金属元素 

分 类 号：R657.31[医药卫生—外科学]