HPLC法测定三种蒙成药中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Three Kinds of Mongolian Recipe By HPLC

作　　者：张学富[1] 那木拉[2] 

机构地区：[1]内蒙古民族大学分析测试中心,内蒙古通辽028042 [2]内蒙古民族大学蒙医药学院

出　　处：《内蒙古民族大学学报（自然科学版）》2006年第5期543-545,共3页Journal of Inner Mongolia Minzu University：Natural Sciences

摘　　要：目的:采用高效液相色谱法测定以黄柏为主要成分的三种蒙成药(协日毛道-8、特木仁-5、协日嘎-4)中盐酸小檗碱的含量.方法:色谱柱为Xterra(5μm×150 mm×3.9 mm)柱;流动相为甲醇-0.02 mol/L磷酸-水-乙腈(10∶30∶30∶30,V/V);检测波长为345 nm.结果:回归方程y=3.408×106x-1.04×105,r=0.9997,CV(%)=1.03(n=5).线性范围0.005 mg/g^5.450 mg/g.协日毛道-8,特木仁-5,协日嘎-4中盐酸小檗碱的含量分别为0.93 mg/g、0.65 mg/g、2.96 mg/g,平均回收率为98.88%.结论:本方法简便、可靠,适合蒙药中盐酸小檗碱的含量测定.Objective： To determine the content of Berberine Hydrochloride in three kinds of Mongolian Recipe by HPLC. Methods： The samples were sparated on a Xterra（5 μm × 150 mm × 3.9 mm） column with methanol - 0.02 mol/L phosphoric acid - water - acetonitrile（10 ＋ 30 ＋ 30 ＋ 30,V/V） as the mobile phase. ;The detection wavelength was 345nm. Results： Regressim equation is y= 3. 408 × 106x - 1.04 × 105, r = 0. 9997,CV（%） = 1.03（n = 5）. The detection limits were in the arange of 0. 005 mg/g- 5. 450 mg/g. The contents of Berberine Hydrochloride in Xirimaodao- 8, Termuren - 5, Xieriga - 4 seperately were 0.93mg/g, 0.65mg/g, 2.96mg/g, The average recovery is 98.88 %. Conclusion： This method was simple, reliable and suitable for the quality control of berberine hydrochloricde in Mongolian Recipe.

关 键 词：蒙成药 高效液相色普法 盐酸小檗碱 含量测定 

分 类 号：R927.2[医药卫生—药学]