辐射交联聚己内酯的酶降解动力学研究  

Kinetic study for enzymatic degradation of PCL

作　　者：张龙彬[1] 朱光明[1] 许硕贵[2] 王金花[1] 秦瑞丰[1] 

机构地区：[1]西北工业大学应用化学系 [2]第二军医大学长海医院,上海200433

出　　处：《辐射研究与辐射工艺学报》2006年第5期275-278,共4页Journal of Radiation Research and Radiation Processing

基　　金：陕西省自然科学基金(2004B05)资助

摘　　要：研究了聚己内酯(Polycaprolactone,PCL)和辐射交联PCL的脂肪酶降解行为及酶促反应动力学,得到了PCL降解速度与时间和底物浓度的关系及Michaelis-Menten常数。实验发现,降解产物浓度随着降解时间和底物浓度增加而增加。PCL粉末和交联PCL颗粒的降解动力学较好地符合米氏方程,其动力学参数Km分别为1.14、71.95mg/mL,最大降解速度Vmax分别为0.39、0.96mL/h。Kinetics of enzymatic degradation behavior of cross-linked PCL was studied. It is well known that the enzymatic hydrolysis of water-insoluble PCL by depolymerases produces water-soluble products. The result showed that the concentration of product in the solution increased with the time and substrates concentration. The degradation kinetics followed Mechaelis-Menten equation. The degradation parameter of the PCL powder was 1.14mg/mL for Km and 0.39mL/h for Vmax, respectively. And the degradation parameter of cross-linked PCL particulate was 71.95mg/mL for Krn and 0.96mL/h for Vmax, respectively.

关 键 词：聚己内酯 辐射交联 酶降解 动力学 

分 类 号：TQ321[化学工程—合成树脂塑料工业]