Study of calf thymus DNA irradiated in vitro with MeV fluorine ions  

作　　者：MIAO Qi LAI Jiang-Nan XUE Jian-Ming QIN Huai-Li WANG Wei-Dong QIN Guang-Yong WANG Yu-Gang 

机构地区：[1]Henan Provincial Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China [2]Key Laboratory of Heavy Ion Physics, Peking University, MOE, Beijing 100871, China

出　　处：《Nuclear Science and Techniques》2006年第5期272-275,共4页核技术（英文）

摘　　要：A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products of calf thymus DNA (CT DNA) irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer (MALDI-TOF), and high-performance liquid chromatography (HPLC). The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel elec- trophoresis, there was no observable product in the range of 1,000 ? 30,000 (m/q) using MALDI-TOF, and small bio- molecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nonrandom.A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects （induced by heavy ions）. In this experiment, the products of calf thymus DNA （CT DNA） irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer （MALDI-TOF）, and high-performance liquid chromatography （HPLC）. The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel electrophoresis, there was no observable product in the range of 1,000- 30,000 （m/q） using MALDI-TOF, and small biomolecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nortrandom.

关 键 词：胸腺DNA 氟离子 体外辐照 DNA损伤机制 

分 类 号：O571.324[理学—粒子物理与原子核物理] Q691.5[理学—物理]