SDF-1对小鼠骨髓源性内皮祖细胞数量及功能的影响  

Effects of SDF-1 on number and biological characteristics of bone marrow derived endothelial progenitor cells in mice

作　　者：尹扬光[1] 黄岚[1] 赵晓辉[1] 方玉强[1] 周健[1] 赵景红[1] 陈剑飞[1] 崔斌[1] 

机构地区：[1]第三军医大学新桥医院全军心血管内科研究所,重庆400037

出　　处：《心血管康复医学杂志》2006年第5期427-430,434,共5页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(项目编号/申请代码:30470729/C03030201)

摘　　要：目的:观察基质细胞衍生因子-1(SDF-1)对体外培养的小鼠骨髓源性内皮祖细胞(EPC s)数量及功能的影响。方法:以密度梯度离心法获取小鼠骨髓单个核细胞(M NC s),由BS-1和D iI-acLDL荧光双染鉴定。加入不同浓度SDF-1 a培养48 h,然后分别采用M TT法、改良的Boyden小室和粘附能力测定来观察EPC s的增殖、迁移和粘附能力;采用血清饥饿法和紫杉醇诱导EPC s凋亡,TUNEL法和流式细胞仪检测SDF-1a对EPC s凋亡率的影响。结果:SDF-1a剂量依赖性增加EPC s数量(P<0.01),并显著改善了EPC s的粘附、迁移和增殖能力(P<0.01),显著降低EPC s凋亡率(P<0.01)。结论:SDF-1a可增加小鼠骨髓源性EPC s的数量并改善EPC s功能。Objective： To study whether SDF-1 has effects on endothelial progenitor cells （EPCs）. Methods： Total mononuclear cells （MNCs） isolated from bone marrow by density gradient centrifugation combined with adherence ceils filtration were plated on fibronectin coated culture dishes. After 7 days, adherent cells were kept with different concentrations of SDF-1a for 48 hours. EPCs proliferation, migration ability and adhesion assay was performed： EPCs apoptosis was induced by paclitaxel or serum starvation for 48h, apoptosis was determined by TUNEL method and flow cytometry. Results ： Incubation of SDF-1a dose dependently increased the number of EPCs （P〈0. 01） ： SDF -1a improved EPCs proliferation, migration and adhesive capacity （P〈0. 01）, and SDF-1a could protect EPCs from paclitaxel or serum starvation-induced apoptosis （P〈0. 01）. Conclusion： SDF-1a can increase the number of bone marrow derived EPCs and improve their biological characteristics.

关 键 词：基质细胞衍生因子-1 内皮细胞 细胞增殖 细胞凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]