高效液相色谱法测定止痒消炎水中蛇床子素含量  

Determination of Osthol in Zhiyangxiaoyan Solution by HPLC

作　　者：李美琴[1] 屈晓萍[1] 李兆奎[1] 

机构地区：[1]浙江省台州市药品检验所,浙江台州318000

出　　处：《中国药业》2006年第18期28-29,共2页China Pharmaceuticals

摘　　要：目的测定止痒消炎水中蛇床子素的含量。方法色谱柱为十八烷基键合硅胶柱,流动相为乙腈-水(60∶40),流速为1mL/min,检测波长为322nm。结果对照品进样量在0.2～1.2μg范围内与峰面积呈良好的线性关系,回归方程Y=4299266X-90914,r=0.9997。平均回收率为100.6%,RSD=1.3%(n=9)。结论高效液相色谱法简便、快速、准确,可作为该制剂的质量控制方法。Objective To establish the determination method for osthol in Zhiyang xiaoyan solution. Methods ODS C18 column was used, acetonitrile-water （60：40） was used as a mobile phase. The detection wavelength was at 322 urn. Results The detection range was 0.2 -1.2μg. There was a good linear relationship between eonsistenee and apex-area. The regress equation is Y=4 299 266X-90 914, r= 0. 999 7. The recovery rate of the added sample was 100.6% and RSD was 1.3% （n =9）. Conclusion This method is simple and the result is reliable.

关 键 词：止痒消炎水 蛇床子素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]